NDLI: An Essential Role for Gamma Interferon in Innate Resistance to Shigella flexneri Infection

Content Provider	PubMed Central
Author	Way, Sing Sing Borczuk, Alain C. Rene, Dominitz Goldberg, Marcia B.
Copyright Year	1998
Abstract	Shigella spp. are the major cause of bacillary dysentery worldwide. To identify immune effectors associated with protection of the naive host during infection, the susceptibility to pulmonary Shigella infection of each of various mouse strains that have a targeted deletion in a specific aspect of the immune system was evaluated. Our results demonstrate that mice deficient in gamma interferon are 5 orders of magnitude more susceptible to Shigella than are wild-type mice, whereas mice deficient in B and T lymphocytes or in T lymphocytes alone exhibit no difference in susceptibility. Significantly lower numbers of shigellae were recovered from immunocompetent compared with gamma-interferon-deficient mice after infection. While immunocompetent mice were able to clear a sublethal Shigella inoculum by day 5 postinfection, progressively increasing numbers of shigellae were cultured from the lungs of gamma interferon-deficient mice over the same period. Histopathology of the lungs from immunocompetent mice infected with a sublethal Shigella inoculum showed mild inflammatory changes, whereas the lungs from gamma interferon-deficient mice demonstrated progressively worsening acute bronchiolitis with ulceration. Further, the time to death in gamma interferon-deficient mice correlates inversely with the size of the Shigella inoculum. To identify the cellular source of gamma interferon, we infected SCID mice, T-cell-receptor-deficient mice, beige mice (a mouse strain deficient in natural killer [NK] cell activity), and mice depleted of NK cells using anti-asialo-GM1. Each NK cell-deficient mouse strain exhibited a 10-fold-greater susceptibility to Shigella infection than immunocompetent mice. To test the protective effects of gamma interferon in vitro, survival of intracellular Shigella was examined in primary macrophages from wild-type mice, primary macrophages from gamma interferon-deficient mice, a macrophage cell line, and a fibroblast cell line. Following activation with gamma interferon, each cell type eradicated intracellular Shigella, while nonactivated macrophages fostered Shigella replication and nonactivated fibroblast cells fostered both Shigella replication and intercellular spread. Taken together, these data establish that NK cell-mediated gamma interferon is essential to resistance following primary Shigella infection.
Starting Page	1342
File Format	PDF
ISSN	10985522
e-ISSN	10985522
Journal	Infection and Immunity
Issue Number	4
Volume Number	66
Language	English
Publisher	American Society for Microbiology
Publisher Date	1998-04-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Parasitology Immunology Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

ShiA Abrogates the Innate T-Cell Response to Shigella flexneri Infection†

Roles for NK Cells and an NK Cell-Independent Source of Intestinal Gamma Interferon for Innate Immunity to Cryptosporidium parvum Infection▿

Shigella flexneri LuxS Quorum-Sensing System Modulates virB Expression but Is Not Essential for Virulence

Role of Intracellular Carbon Metabolism Pathways in Shigella flexneri Virulence

Gamma Interferon Is Essential for Clearing Mycobacterium genavense Infection

Role and Regulation of the Shigella flexneri Sit and MntH Systems

Infection of Cultured Mouse Macrophages with Shigella flexneri

Role of gamma interferon and tumor necrosis factor alpha in resistance to Salmonella typhimurium infection.

Role and Regulation of Iron-Sulfur Cluster Biosynthesis Genes in Shigella flexneri Virulence▿

An Essential Role for Gamma Interferon in Innate Resistance to Shigella flexneri Infection

Similar Documents

ShiA Abrogates the Innate T-Cell Response to Shigella flexneri Infection†

Roles for NK Cells and an NK Cell-Independent Source of Intestinal Gamma Interferon for Innate Immunity to Cryptosporidium parvum Infection▿

Shigella flexneri LuxS Quorum-Sensing System Modulates virB Expression but Is Not Essential for Virulence

Role of Intracellular Carbon Metabolism Pathways in Shigella flexneri Virulence

Gamma Interferon Is Essential for Clearing Mycobacterium genavense Infection

Role and Regulation of the Shigella flexneri Sit and MntH Systems

Infection of Cultured Mouse Macrophages with Shigella flexneri

Role of gamma interferon and tumor necrosis factor alpha in resistance to Salmonella typhimurium infection.

Role and Regulation of Iron-Sulfur Cluster Biosynthesis Genes in Shigella flexneri Virulence▿

An Essential Role for Gamma Interferon in Innate Resistance to Shigella flexneri Infection