NDLI: A Complex Control System for Transcriptional Activation from the sid Promoter of Bacteriophage P4

Content Provider	PubMed Central
Author	Reiter, Kaye Lam, Hugh Young, Edward Julien, Bryan Richard, Calendar
Copyright Year	1998
Abstract	The sid gene promoter (P sid ), which controls expression of the late genes from satellite phage P4, is activated by a unique class of small DNA-binding proteins. The activators from both satellite and helper phages stimulate transcription from P sid . These activators bind to sites centered at position −55 in all the helper and satellite phage late promoters. P4 P sid is unique in that it has an additional activator binding site centered at position −18 (site II). We have constructed a mutant of site II that no longer binds activators. Transcription under the control of satellite phage activators is increased by the site II mutation. In contrast, helper phage activators do not show this increase in transcription from P sid mutated at site II. Competition gel shift analysis reveals that the P4 satellite phage activator, Delta, binds eightfold better to site II than to site I. The products of the sid transcription unit are needed only when a helper phage is present; thus, the satellite phage activators repress transcription until the helper is present to supply a nonrepressing activator.
Starting Page	5151
File Format	PDF
ISSN	10985530
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	19
Volume Number	180
Language	English
Publisher	American Society for Microbiology
Publisher Date	1998-10-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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