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| Content Provider | PubMed Central |
|---|---|
| Author | Keifer, David W. Franceschi, Vincent R. Lucas, William J. |
| Abstract | Chloride transport, presumably via a Cl−-2H+ co-transport system, was investigated in Chara corallina. At pH 6.5, the control influx (3.1 picomoles per centimeter2 per second) was stimulated 4-fold by an 18-hour Cl− starvation. The stimulated influx was inhibited to 4.7 picomoles per centimeter2 per second after a 60-minute pre-exposure to 0.5 millimolar 4,4′-diisothiocyano-2,2′-disulfonic acid stilbene (DIDS). This compares with a nonsignificant inhibition of the control under similar conditions. At 2 millimolar DIDS, both stimulated and control influx were inhibited to values of 1.1 and 2.2 picomoles per centimeter2 per second, respectively; in all cases, DIDS inhibition was reversible. Over the pH range 4.8 to 8.5, the control and DIDS-inhibited influx showed only slight pH sensitivity; in contrast, the stimulated flux was strongly pH dependent (pH 6.5 optimum). Inasmuch as changes in pH alter membrane potential, N-ethylmaleimide was used to depolarize the membrane; this had no effect on Cl− influx. A transient depolarization of the membrane (about 20 millivolts) was observed on restoration of Cl− to starved cells. The membrane also depolarized transiently when starved cells were exposed to 0.5 millimolar DIDS, but the depolarization associated with Cl− restoration was inhibited by a 40-minute pretreatment with DIDS. Exposure of control cells to DIDS caused only a small hyperpolarization (about 7 millivolts). DIDS may have blocked Cl− influx by inhibiting the putative plasmalemma H+-translocating ATPase. Histochemical studies on intact cells revealed no observable effect of DIDS on plasmalemma ATPase activity. However, DIDS application after fixation resulted in complete inhibition of ATPase activity. |
| Starting Page | 1327 |
| File Format | |
| ISSN | 15322548 |
| e-ISSN | 15322548 |
| Journal | Plant Physiology |
| Issue Number | 5 |
| Volume Number | 70 |
| Language | English |
| Publisher Date | 1982-11-01 |
| Access Restriction | Open |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Physiology Plant Science |
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