NDLI: Genomic Analysis of Bacteriophage ΦJL001: Insights into Its Interaction with a Sponge-Associated Alpha-Proteobacterium†

Content Provider	PubMed Central
Author	Lohr, Jayme E. Chen, Feng Hill, Russell T.
Copyright Year	2005
Abstract	Bacteriophage ΦJL001 infects a novel marine bacterium in the α subclass of the Proteobacteria isolated from the marine sponge Ircinia strobilina. ΦJL001 is a siphovirus and forms turbid plaques on its host. The genome sequence of ΦJL001 was determined in order to better understand the interaction between the marine phage and its sponge-associated host bacterium. The complete genome sequence of ΦJL001 comprised 63,469 bp with an overall G+C content of 62%. The genome has 91 predicted open reading frames (ORFs), and 17 ORFs have been assigned putative functions. ΦJL001 appears to be a temperate phage, and the integrase gene was identified in the genome. DNA hybridization analysis showed that the ΦJL001 genome does not integrate into the host chromosome under the conditions tested. DNA hybridization experiments therefore suggested that ΦJL001 has some pseudolysogenic characteristics. The genome of ΦJL001 contains many putative genes involved in phage DNA replication (e.g., helicase, DNA polymerase, and thymidylate synthase genes) and also contains a putative integrase gene associated with the lysogenic cycle. Phylogeny based on DNA polymerase gene sequences indicates that ΦJL001 is related to a group of siphoviruses that infect mycobacteria. Designation of ΦJL001 as a siphovirus is consistent with the morphology of the phage visualized by transmission electron microscopy. The unique marine phage-host system described here provides a model system for studying the role of phages in sponge microbial communities.
Related Links	http://dx.doi.org/10.1128/aem.71.3.1598-1609.2005
Ending Page	1609
Page Count	12
Starting Page	1598
File Format	PDF
ISSN	00992240
e-ISSN	10985336
Journal	Applied and Environmental Microbiology
Issue Number	3
Volume Number	71
Language	English
Publisher	American Society for Microbiology
Publisher Date	2005-03-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Biotechnology Food Science Ecology Applied Microbiology and Biotechnology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ecology Food Science Applied Microbiology and Biotechnology Biotechnology

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