NDLI: phnE and glpT Genes Enhance Utilization of Organophosphates in Escherichia coli K-12

Content Provider	PubMed Central
Author	Ilya, Elashvili Defrank, Joseph J. Culotta, Valeria C.
Copyright Year	1998
Abstract	Wild-type Escherichia coli K-12 strain JA221 grows poorly on low concentrations (≤1 mM) of diisopropyl fluorophosphate and its hydrolysis product, diisopropyl phosphate (DIPP), as sole phosphorus sources. Spontaneous organophosphate utilization (OPU) mutants were isolated that efficiently utilized these alternate sources of phosphate. A genomic library was constructed from one such OPU mutant, and two genes were isolated that conferred the OPU phenotype to strain JA221 upon transformation. These genes were identified as phnE and glpT. The original OPU mutation represented phnE gene activation and corresponded to the same 8-bp unit deletion from the cryptic wild-type E. coli K-12 phnE gene that has been shown previously to result in phnE activation. In comparison, sequence analysis revealed that the observed OPU phenotype conferred by the glpT gene was not the result of a mutation. PCR clones of glpT from both the mutant and the wild type were found to confer the OPU phenotype to JA221 when they were present on the high-copy-number pUC19 plasmid but not when they were present on the low-copy-number pWSK29 plasmid. This suggests that the OPU phenotype associated with the glpT gene is the result of amplification and overproduction of the glpT gene product. Both the active phnE and multicopy glpT genes facilitated effective metabolism of low concentrations of DIPP, whereas only the active phnE gene could confer the ability to break down a chromogenic substrate, 5-bromo-4-chloro-3-indoxyl phosphate-p-toluidine (X-Pi). This result indicates that in E. coli, X-Pi is transported exclusively by the Phn system, whereas DIPP (or its metabolite) may be transported by both Phn and Glp systems.
Starting Page	2601
File Format	PDF
ISSN	10985336
e-ISSN	10985336
Journal	Applied and Environmental Microbiology
Issue Number	7
Volume Number	64
Language	English
Publisher	American Society for Microbiology
Publisher Date	1998-07-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ecology Food Science Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Deletion of Genes Encoding Cytochrome Oxidases and Quinol Monooxygenase Blocks the Aerobic-Anaerobic Shift in Escherichia coli K-12 MG1655 ▿ †

Acquisition of a sucrose utilization system in Escherichia coli K-12 derivatives and its application to industry.

Rapid and accurate identification of Escherichia coli K-12 strains.

Transcriptional Responses of Escherichia coli K-12 and O157:H7 Associated with Lettuce Leaves

Transcriptional Responses of Escherichia coli K-12 and O157:H7 Associated with Lettuce Leaves

Reductive dehalogenation of carbon tetrachloride by Escherichia coli K-12.

Heat damage to the chromosome of Escherichia coli K-12.

Nickel Promotes Biofilm Formation by Escherichia coli K-12 Strains That Produce Curli▿

Construction of an Escherichia coli K-12 Mutant for Homoethanologenic Fermentation of Glucose or Xylose without Foreign Genes▿

phnE and glpT Genes Enhance Utilization of Organophosphates in Escherichia coli K-12

Similar Documents

Deletion of Genes Encoding Cytochrome Oxidases and Quinol Monooxygenase Blocks the Aerobic-Anaerobic Shift in Escherichia coli K-12 MG1655 ▿ †

Acquisition of a sucrose utilization system in Escherichia coli K-12 derivatives and its application to industry.

Rapid and accurate identification of Escherichia coli K-12 strains.

Transcriptional Responses of Escherichia coli K-12 and O157:H7 Associated with Lettuce Leaves

Transcriptional Responses of Escherichia coli K-12 and O157:H7 Associated with Lettuce Leaves

Reductive dehalogenation of carbon tetrachloride by Escherichia coli K-12.

Heat damage to the chromosome of Escherichia coli K-12.

Nickel Promotes Biofilm Formation by Escherichia coli K-12 Strains That Produce Curli▿

Construction of an Escherichia coli K-12 Mutant for Homoethanologenic Fermentation of Glucose or Xylose without Foreign Genes▿

phnE and glpT Genes Enhance Utilization of Organophosphates in Escherichia coli K-12