NDLI: Oxygen-Dependent Regulation of the Expression of the Catalase Gene katA of Lactobacillus sakei LTH677

Content Provider	PubMed Central
Author	Hertel, Christian Schmidt, Gudrun Fischer, Marc Oellers, Katja Hammes, Walter P.
Copyright Year	1998
Abstract	The catalase gene katA of Lactobacillus sakei LTH677 was cloned and expressed in Escherichia coli UM2, Lactobacillus casei LK1, and Lactobacillus curvatus LTH1432. The last host is a catalase-deficient plasmid-cured derivative of a starter organism used in meat fermentation. The regulation of katA expression was found to be the same in L. sakei LTH677 and the recombinant strains. The addition of H2O2 to anaerobic cultures, as well as a switch to aerobic conditions, resulted in a strong increase in KatA activity. The expression was investigated in more detail with L. sakei LTH677 and L. curvatus LTH4002. The recombinant strain LTH4002 did not accumulate H2O2 under glucose-limited aerobic conditions and remained viable in the stationary phase. Under inductive conditions, the katA-specific mRNA and the apoenzyme were synthesized de novo. Deletion derivatives of the katA promoter were produced, and the regulatory response was investigated by fusion to the β-glucuronidase reporter gene gusA and expression in L. sakei LTH677. The fact that gene expression was subject to induction was confirmed at the level of transcription and protein synthesis. A small putative regulatory sequence of at least 25 bp was identified located upstream of the −35 site. Competition experiments performed with L. sakei LTH677 harboring the fusion constructs consisting of the katA promoter and gusA revealed that an activator protein is involved in the transcriptional induction of katA.
Starting Page	1359
File Format	PDF
ISSN	10985336
e-ISSN	10985336
Journal	Applied and Environmental Microbiology
Issue Number	4
Volume Number	64
Language	English
Publisher	American Society for Microbiology
Publisher Date	1998-04-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ecology Food Science Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Cloning, sequence, and phenotypic expression of katA, which encodes the catalase of Lactobacillus sake LTH677.

High-Level Expression of Heme-Dependent Catalase Gene katA from Lactobacillus Sakei Protects Lactobacillus Rhamnosus from Oxidative Stress

Expression of the Arginine Deiminase Pathway Genes in Lactobacillus sakei Is Strain Dependent and Is Affected by the Environmental pH

The Product of arcR, the Sixth Gene of the arc Operon of Lactobacillus sakei, Is Essential for Expression of the Arginine Deiminase Pathway

Cloning and Heterologous Expression of Hematin-Dependent Catalase Produced by Lactobacillus plantarum CNRZ 1228

Identification, Characterization, and Expression of a Second, Bicistronic, Operon Involved in the Production of Lactocin S in Lactobacillus sakei L45

Purification and Characterization of an Arginine Aminopeptidase from Lactobacillus sakei

The pepR Gene of Lactobacillus sakei Is Positively Regulated by Anaerobiosis at the Transcriptional Level

Purification and Characterization of an X-Prolyl-Dipeptidyl Peptidase from Lactobacillus sakei

Oxygen-Dependent Regulation of the Expression of the Catalase Gene katA of Lactobacillus sakei LTH677

Similar Documents

Cloning, sequence, and phenotypic expression of katA, which encodes the catalase of Lactobacillus sake LTH677.

High-Level Expression of Heme-Dependent Catalase Gene katA from Lactobacillus Sakei Protects Lactobacillus Rhamnosus from Oxidative Stress

Expression of the Arginine Deiminase Pathway Genes in Lactobacillus sakei Is Strain Dependent and Is Affected by the Environmental pH

The Product of arcR, the Sixth Gene of the arc Operon of Lactobacillus sakei, Is Essential for Expression of the Arginine Deiminase Pathway

Cloning and Heterologous Expression of Hematin-Dependent Catalase Produced by Lactobacillus plantarum CNRZ 1228

Identification, Characterization, and Expression of a Second, Bicistronic, Operon Involved in the Production of Lactocin S in Lactobacillus sakei L45

Purification and Characterization of an Arginine Aminopeptidase from Lactobacillus sakei

The pepR Gene of Lactobacillus sakei Is Positively Regulated by Anaerobiosis at the Transcriptional Level

Purification and Characterization of an X-Prolyl-Dipeptidyl Peptidase from Lactobacillus sakei

Oxygen-Dependent Regulation of the Expression of the Catalase Gene katA of Lactobacillus sakei LTH677