NDLI: Interference of Phenolic Compounds with the 1-Aminocyclopropane-1-Carboxylic Acid Assay 1

Content Provider	PubMed Central
Author	Yaron, Sitrit Joseph, Riov Blumenfeld, Amos
Abstract	The yields of ethylene from endogenous and exogenous 1-aminocyclo-propane-1-carboxylic acid (ACC) in avocado (Persea Americana Mill.) fruit pedicel extracts were very low when assayed by the method of Lizada and Yang (1979 Anal Biochem 100: 140-145). Addition of phenolic compounds, which are present in avocado tissues, to the assay mixture significantly reduced the conversion efficiency of ACC to ethylene. A negative correlation was found between the amount of the plant material in the assay mixture and the conversion efficiency of ACC to ethylene. Removal of phenolic compounds from pedicel extracts by polyvinylpolypyrrolidone, Amberlite XAD-7, and Dowex-50 column chromatography or lead acetate precipitation greatly increased the yields of thylene from ACC in these extracts. The use of polyvinylpolypyrrolidone column chromatography also enabled us to obtain more accurate estimations of endogenous ACC levels in carnation (Dianthus caryophyllus L.) petal extracts. The conversion efficiency of ACC to ethylene could be improved by increasing the concentrations of mercuric chloride and NaOCl in the assay mixture.
Starting Page	13
File Format	PDF
ISSN	15322548
e-ISSN	15322548
Journal	Plant Physiology
Issue Number	1
Volume Number	86
Language	English
Publisher Date	1988-01-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics Physiology Plant Science

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Interference of Phenolic Compounds with the 1-Aminocyclopropane-1-Carboxylic Acid Assay 1