NDLI: Functional Importance of the Coiled-Coil of the Ebola Virus Glycoprotein

Content Provider	PubMed Central
Author	Watanabe, Shinji Takada, Ayato Watanabe, Tokiko Ito, Hiroshi Kida, Hiroshi Kawaoka, Yoshihiro
Copyright Year	2000
Abstract	Ebola virus contains a single glycoprotein (GP) that is responsible for receptor binding and membrane fusion and is proteolytically cleaved into disulfide-linked GP1 and GP2 subunits. The GP2 subunit possesses a coiled-coil motif, which plays an important role in the oligomerization and fusion activity of other viral GPs. To determine the functional significance of the coiled-coil motif of GP2, we examined the effects of peptides corresponding to the coiled-coil motif of GP2 on the infectivity of a mutant vesicular stomatitis virus (lacking the receptor-binding/fusion protein) pseudotyped with the Ebola virus GP. A peptide corresponding to the C-terminal helix reduced the infectivity of the pseudotyped virus. We next introduced alanine substitutions into hydrophobic residues in the coiled-coil motif to identify residues important for GP function. None of the substitutions affected GP oligomerization, but some mutations, two in the N-terminal helix and all in the C-terminal helix, reduced the ability of GP to confer infectivity to the mutant vesicular stomatitis virus without affecting the transport of GP to the cell surface, its incorporation into virions, and the production of virus particles. These results indicate that the coiled-coil motif of GP2 plays an important role in facilitating the entry of Ebola virus into host cells and that peptides corresponding to this region could act as efficient antiviral agents.
Starting Page	10194
File Format	PDF
ISSN	10985514
e-ISSN	10985514
Journal	Journal of Virology
Issue Number	21
Volume Number	74
Language	English
Publisher	American Society for Microbiology
Publisher Date	2000-11-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Virology Immunology Microbiology Insect Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

An Antiviral Peptide Targets a Coiled-Coil Domain of the Human T-Cell Leukemia Virus Envelope Glycoprotein

Covalent Modifications of the Ebola Virus Glycoprotein

Infectivity-Enhancing Antibodies to Ebola Virus Glycoprotein

Role of Endosomal Cathepsins in Entry Mediated by the Ebola Virus Glycoprotein

A Heterologous Coiled Coil Can Substitute for Helix I of the Sindbis Virus Capsid Protein

Proteolytic Processing of the Ebola Virus Glycoprotein Is Not Critical for Ebola Virus Replication in Nonhuman Primates▿

Functional Mapping of the Nucleoprotein of Ebola Virus

A New Ebola Virus Nonstructural Glycoprotein Expressed through RNA Editing ▿

The Core of the Respiratory Syncytial Virus Fusion Protein Is a Trimeric Coiled Coil

Functional Importance of the Coiled-Coil of the Ebola Virus Glycoprotein

Similar Documents

An Antiviral Peptide Targets a Coiled-Coil Domain of the Human T-Cell Leukemia Virus Envelope Glycoprotein

Covalent Modifications of the Ebola Virus Glycoprotein

Infectivity-Enhancing Antibodies to Ebola Virus Glycoprotein

Role of Endosomal Cathepsins in Entry Mediated by the Ebola Virus Glycoprotein

A Heterologous Coiled Coil Can Substitute for Helix I of the Sindbis Virus Capsid Protein

Proteolytic Processing of the Ebola Virus Glycoprotein Is Not Critical for Ebola Virus Replication in Nonhuman Primates▿

Functional Mapping of the Nucleoprotein of Ebola Virus

A New Ebola Virus Nonstructural Glycoprotein Expressed through RNA Editing ▿

The Core of the Respiratory Syncytial Virus Fusion Protein Is a Trimeric Coiled Coil

Functional Importance of the Coiled-Coil of the Ebola Virus Glycoprotein