NDLI: Variations in enzymatic hydrolysis efficiencies for amitriptyline and cyclobenzaprine in urine

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Variations in enzymatic hydrolysis efficiencies for amitriptyline and cyclobenzaprine in urine

Content Provider	Paperity
Author	Mastrianni, Kaylee R. Lee, L. Andrew Barna, Michael Brewer, William E. Dongari, Nagaraju Morgan, Stephen L.
Abstract	A collaborative study was conducted to investigate discrepancies in recoveries of two commonly prescribed compounds, amitriptyline and cyclobenzaprine, in patient urine samples when hydrolyzed with different enzymes from different sources. A 2- to 10-fold increase in analyte recoveries was seen for patient samples hydrolyzed using a recombinant Î²-glucuronidase (IMCSzymeâ„¢) over samples hydrolyzed with Î²-glucuronidase from Haliotis rufescens. We report outcomes from four commercially available Î²-glucuronidase enzymes (IMCSzymeâ„¢, Patella vulgata, Helix pomatia and H. rufescens) on patient samples that tested positive for amitriptyline and cyclobenzaprine. Our results confirm reduced hydrolysis of glucuronides by Î²-glucuronidases isolated from mollusks, but near complete conversion when using the recombinant enzyme. Our premise is that systematic differences in hydrolysis efficiencies due to varying substrate affinity among enzyme subtypes potentially impacts accuracy and reliability of measurements.
Starting Page	732
Ending Page	737
File Format	HTM / HTML
ISSN	01464760
DOI	10.1093/jat/bkw062
Issue Number	9
Journal	Journal of Analytical Toxicology
Volume Number	40
e-ISSN	19452403
Language	English
Publisher	Oxford University Press
Publisher Date	2016-11-16
Access Restriction	Open
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Health, Toxicology and Mutagenesis Analytical Chemistry Toxicology Chemical Health and Safety

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