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Moringa oleifera aqueous leaf extract inhibits reducing monosaccharide-induced protein glycation and oxidation of bovine serum albumin
| Content Provider | Paperity |
|---|---|
| Author | Adisakwattana, Sirichai Dahlan, Winai Nunthanawanich, Pornpimon Mäkynen, Kittana Ngamukote, Sathaporn Sirikwanpong, Sukrit Sompong, Weerachat |
| Abstract | Advanced glycation end products (AGEs) play an important factor for pathophysiology of diabetes and its complications. Moringa oleifera is one of the medicinal plants that have anti-hyperglycemic activity. However, anti-glycation property of Moringa oleifera leaf extract on the different types of reducing monosaccharides-induced protein glycation has not been investigated. Therefore, the aim of this study was to examine the protective effect of Moringa oleifera aqueous leaf extract (MOE) on reducing sugars-induced protein glycation and protein oxidation. Total phenolic content of MOE was measured using the Folin–Ciocalteu method. Bovine serum albumin was incubated with 0.5 M of reducing sugars (glucose or fructose) with or without MOE (0.5–2.0 mg/mL) for 1, 2, 3 and 4 weeks. The results found that total phenolic content was 38.56 ± 1.50 mg gallic acid equivalents/g dry extract. The formation of fluorescent and non-fluorescent AGEs [N ε-(carboxymethyl) lysine (CML)] and the level of fructosamine were determined to indicate protein glycation, whereas the level of protein carbonyl content and thiol group were examined for protein oxidation. MOE (0.5–2.0 mg/mL) significantly inhibited the formation of fluorescent, N ε-CML and markedly decreased fructosamine level (P < 0.05). Moreover, MOE significantly prevented protein oxidation manifested by reducing protein carbonyl and the depletion of protein thiol in a dose-dependent manner (P < 0.05). Thus, the findings indicated that polyphenols containing in MOE have high potential for decreasing protein glycation and protein oxidation that may delay or prevent AGE-related diabetic complications. |
| Starting Page | 1098 |
| File Format | HTM / HTML |
| DOI | 10.1186/s40064-016-2759-3 |
| Issue Number | 1 |
| Journal | SpringerPlus |
| Volume Number | 5 |
| e-ISSN | 21931801 |
| Language | English |
| Publisher | Springer International Publishing |
| Publisher Date | 2016-07-16 |
| Access Restriction | Open |
| Subject Keyword | Polyphenol Moringa oleifera Glucose Glycation Fructose |
| Content Type | Text |
| Resource Type | Article |
