NDLI: Scorpion toxin BmK I directly activates Nav1.8 in primary sensory neurons to induce neuronal hyperexcitability in rats

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Scorpion toxin BmK I directly activates Nav1.8 in primary sensory neurons to induce neuronal hyperexcitability in rats

Content Provider	Paperity
Author	Li, Zhenwei Hua, Liming Jiao, Yunlu Fu, Jin Liu, Tong Ji, Yonghua Ye, Pin Jiang, Feng
Abstract	Voltage-gated sodium channels (VGSCs) in primary sensory neurons play a key role in transmitting pain signals to the central nervous system. BmK I, a site-3 sodium channel-specific toxin from scorpion Buthus martensi Karsch, induces pain behaviors in rats. However, the subtypes of VGSCs targeted by BmK I were not entirely clear. We therefore investigated the effects of BmK I on the current amplitude, gating and kinetic properties of Nav1.8, which is associated with neuronal hyperexcitability in DRG neurons. It was found that BmK I dose-dependently increased Nav1.8 current in small-sized (<25 μm) acutely dissociated DRG neurons, which correlated with its inhibition on both fast and slow inactivation. Moreover, voltage-dependent activation and steady-state inactivation curves of Nav1.8 were shifted in a hyperpolarized direction. Thus, BmK I reduced the threshold of neuronal excitability and increased action potential firing in DRG neurons. In conclusion, our data clearly demonstrated that BmK I modulated Nav1.8 remarkably, suggesting BmK I as a valuable probe for studying Nav1.8. And Nav1.8 is an important target related to BmK I-evoked pain.
Starting Page	443
Ending Page	452
File Format	HTM / HTML
ISSN	1674800X
DOI	10.1007/s13238-015-0154-4
Issue Number	6
Journal	Protein & Cell
Volume Number	6
e-ISSN	16748018
Language	English
Publisher	SpringerOpen
Publisher Date	2015-06-01
Access Restriction	Open
Subject Keyword	Primary sensory neurons Bmk i Voltage-gated sodium channel Nav1.8
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Drug Discovery Biochemistry Biotechnology

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Scorpion toxin BmK I directly activates Nav1.8 in primary sensory neurons to induce neuronal hyperexcitability in rats

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Scorpion toxin BmK I directly activates Nav1.8 in primary sensory neurons to induce neuronal hyperexcitability in rats

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Scorpion toxin BmK I directly activates Nav1.8 in primary sensory neurons to induce neuronal hyperexcitability in rats

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