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Mice deficient in intestinal γδ intraepithelial lymphocytes display an altered intestinal O-glycan profile compared with wild-type littermates
| Content Provider | Paperity |
|---|---|
| Author | Olivia, I. Kober Fuell, Christine Juge, Nathalie Hautefort, Isabelle |
| Abstract | Intestinal γδ T-cell receptor-bearing intraepithelial lymphocytes (γδ IELs) play a multifaceted role in maintaining mucosal homeostasis. In order to investigate the relationship between O-glycosylation and inflammation, we carried out an in-depth mass spectrometric comparison of the intestinal O-glycosylation profile of mice lacking γδ IELs (TCRδ−/−) and of their wild-type (WT) littermates. A total of 69 nonsulfated and 59 sulfated compositional types of O-glycans were identified in the small intestine and colon of TCRδ−/− and WT mice. Our results demonstrated structural differences in intestinal glycosylation in TCRδ−/− mice compared with WT littermates. TCRδ−/− colons contained a lower proportion of core-2 structures and an increased proportion of core-1 structures whereas TCRδ−/− small intestines had a decreased percentage of core-3 structures. The glycan antennae in TCRδ−/− colon and small intestine showed altered structural diversity compared with WT mice. There were significant differences in the sialylated species between the TCRδ−/− and WT mice with the sialylated Tn antigen found exclusively in the TCRδ−/−small intestine, whereas the sulfation pattern remained mostly unchanged. These findings provide novel molecular insights underpinning the role of γδ IELs in maintaining gut homeostasis. |
| Starting Page | 42 |
| Ending Page | 54 |
| File Format | HTM / HTML |
| ISSN | 09596658 |
| DOI | 10.1093/glycob/cwu088 |
| Issue Number | 1 |
| Journal | Glycobiology |
| Volume Number | 25 |
| e-ISSN | 14602423 |
| Language | English |
| Publisher | Oxford University Press |
| Publisher Date | 2015-01-01 |
| Access Restriction | Open |
| Subject Keyword | O-glycosylation Intestinal intraepithelial t lymphocytes Mouse intestine Maldi-tof-ms T-cell receptor-î³î´ |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry |
