NDLI: Oxidized low density lipoprotein stimulates aortic smooth muscle cell proliferation

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Oxidized low density lipoprotein stimulates aortic smooth muscle cell proliferation

Content Provider	Paperity
Author	Ghosh, Nupur Chatterjee, Subroto
Abstract	We have investigated the effects of oxidized low density lipoproteins (Ox-LDL) on aortic smooth muscle cell (SMC) proliferation and the biosynthesis of glycosphingo-lipids. We found that Ox-LDL exerted a concentration, time, and temperature dependent alteration of cell proliferation and the biosynthesis of lactosylceramide. At low concentrations (5â€“10 Î¼g/ml medium) Ox-LDL stimulated cell proliferation measured by an increase in the incorporation of 3H-thymidine in cells and the synthesis of lactosylceramide, but not glucosylceramide synthesis. Oxidized LDL exerted a threefold increase in the incorporation of [3H]-galactose and [3H]-serine in lactosylceramide. The activity of lactosylceramide synthetase; UDP-galactose glucosylceramide Î²1 â†’ 4 galactosyltransferase (GaTT-2), but not glucosylceramide synthetase (GlcT-1) was stimulated by Ox-LDL. On the other hand, LDL suppressed the activity of GaIT-2 in these cells. When cells were preincubated with antibody against Ox-LDL or GaIT-2 it compromised the Ox-LDL mediated stimulation in cell proliferation and GaIT-2 activity. Similarly, D-PDMP an inhibitor of GaIT-2 compromised the Ox-LDL mediated effects in cells. In contrast, L-PDMP further stimulated the Ox-LDL mediated cell proliferation and GaIT-2 activity. However, preincubation of cells with preimmune rabbit serum IgG failed to abrogate Ox-LDL mediated stimulation in cell proliferation and GaIT-2 activity. In sum, we found that Ox-LDL stimulated aortic smooth muscle cell proliferation in culture. This effect resulted from Ox-LDL mediated activation of GaIT-2 that produced lactosylceramide. Lactosylceramide in turn, contributed to cell proliferation. Such correlations are supportive of the notion that GaIT-2 action mediates the signal transduction of Ox-LDL contributing to cell proliferation.
Starting Page	303
Ending Page	311
File Format	HTM / HTML
ISSN	09596658
DOI	10.1093/glycob/6.3.303
Issue Number	3
Journal	Glycobiology
Volume Number	6
e-ISSN	14602423
Language	English
Publisher	Oxford University Press
Publisher Date	1996-04-01
Access Restriction	Open
Subject Keyword	Glucosylceramide (clccer) Lactosylceramide (laccer) Oxidized low density lipoproteins (ox-ldl)
Content Type	Text
Resource Type	Article
Subject	Biochemistry

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