NDLI: A novel 4-methylumbelliferyl-β-D-xyloside derivative, sulfate-O-3-xylosylβ1-(4-methylumbelliferone), isolated from culture medium of human skin fibroblasts, and its role in methylumbelliferone-initiated glycosaminoglycan biosynthesis

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A novel 4-methylumbelliferyl-β-D-xyloside derivative, sulfate-O-3-xylosylβ1-(4-methylumbelliferone), isolated from culture medium of human skin fibroblasts, and its role in methylumbelliferone-initiated glycosaminoglycan biosynthesis

Content Provider	Paperity
Author	Matsuya, Hideki Nakamura, Toshiya Sasaki, Mutsuo Takagaki, Keiichi Tazawa, Toshiyuki Endo, Masahiko
Abstract	Human skin fibroblasts were incubated in the presence of 4-methylumbelliferyl-Î²-d-xyloside (Xyl-MU). The culture medium was recovered and Xyl-MU derivatives which were initiated by the Xyl-MU acting as a primer were purified. As a result, a novel Xyl-MU derivative was isolated, in addition to previously reported Xyl-MU derivatives such as glycosaminoglycan-MU, Gal-Gal-Xyl-MU, Gal-Xyl-MU, SA-Gal-Xyl-MU, Xyl-Xyl-MU, GlcA-Xyl-MU, and sulfate-GlcA-Xyl-MU. This Xyl-MU derivative was subjected to carbohydrate composition analysis, enzyme digestion, ion-spray mass spectrometric analysis, and Smith degradation. The results indicated that it was sulfate-O-3-Xyl-MU. When Xyl-MU was incubated with [35S]PAPS using a homogenate prepared from the same cultured skin fibroblasts, [35S]sulfate-O-3-Xyl-MU was produced. Moreover, when Xyl-MU was incubated with UDP-[3H]Gal, [3H]galactose was transferred to Xyl-MU, but when sulfate-O-3-Xyl-MU was incubated with UDP-[3H]Gal, [3H]galactose was not transferred. These results indicate that chain elongation from Xyl-MU is inhibited by sulfation of Xyl-MU, and that Xyl-MU sulfation is involved in the control of Xyl-MU-initiated glycosaminoglycan biosynthesis.
Starting Page	879
Ending Page	884
File Format	HTM / HTML
ISSN	09596658
Issue Number	9
Journal	Glycobiology
Volume Number	8
e-ISSN	14602423
Language	English
Publisher	Oxford University Press
Publisher Date	1998-09-01
Access Restriction	Open
Subject Keyword	Glycosaminoglycan biosynthesis 4-methylumbelliferyl-b-d-xyloside Sulfate-o-3-xylosylb1-(4-methylumbelliferone) Human skin fibroblasts
Content Type	Text
Resource Type	Article
Subject	Biochemistry

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