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Production of dihydroxyacetone from an aqueous solution of glycerol in the reaction catalyzed by an immobilized cell preparation of acetic acid bacteria Gluconobacter oxydans ATCC 621
| Content Provider | Paperity |
|---|---|
| Author | Stanisław, Błażejak Lidia, Stasiak-różańska |
| Abstract | Dihydroxyacetone (1,3-dihydroxy-2-propanone, DHA) is applied in the food and cosmetic industries as well as in pharmacy and medicine. It is produced as a result of incomplete oxidation of glycerol by acetic acid bacteria Gluconobacter oxydans. This reaction is catalyzed by PQQ-dependent membrane-bound glycerol dehydrogenase. The research developed a method of obtaining DHA by oxidation of a 3 % aqueous solution of glycerol (pH 7.5) at a temperature of 23 °C, with the only reaction biocatalyst being an immobilized cell preparation obtained from G. oxydans cells. After 5 days of the process, DHA concentration in the solution accounted for 27.2 g/L and the reaction efficiency for 94 %. After 4 days of the reaction run in culture media with pH 5.0, at a temperature of 28 °C, free or immobilized cells of G. oxydans produced on average 25 g of DHA/L at the reaction efficiency of 87 %. |
| Starting Page | 1125 |
| Ending Page | 1132 |
| File Format | HTM / HTML |
| ISSN | 14382377 |
| DOI | 10.1007/s00217-012-1846-0 |
| Issue Number | 6 |
| Journal | European Food Research and Technology |
| Volume Number | 235 |
| e-ISSN | 14382385 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2012-10-09 |
| Access Restriction | Open |
| Subject Keyword | Glycerol Glycerol dehydrogenase Immobilization Gluconobacter oxydans Cell preparation Dihydroxyacetone |
| Content Type | Text |
| Resource Type | Article |
| Subject | Industrial and Manufacturing Engineering Chemistry Biochemistry Food Science Biotechnology |
