NDLI: Caracterización de los mecanismos de resistencia a azoles en aislados clínicos chilenos de Candida albicans

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Caracterización de los mecanismos de resistencia a azoles en aislados clínicos chilenos de Candida albicans

Content Provider	Paperity
Author	Alburquenque, Claudio Fuentes, Marisol Hermosilla, Germán Mary, A. Falconer Tapia, Cecilia Amaro, José
Abstract	Introduction: The commensal yeast Candida albicans, can cause superficial or systemic candidiasis in susceptible hosts. In Chile, azole antifungals are the most widely used drugs in the treatment of candidiasis. In a previous study performed at our center, 2.1 and 1.6% of clinical isolates of C. albicans were found to be resistant to fluconazole and voriconazole, respectively. Objective: To characterize the resistance mechanisms involved in azoles resistance in Chilean clinical isolates. Methodology: Eight resistant, nine susceptible-dose dependent (SDD) and 10 susceptible strains (n: 27) were selected according to the Clinical Laboratory Standards Institute (CLSI) M27-S3 criteria, from vaginal and urine samples. Mutations in the 408-488 region of the ERG11 gene were studied by sequencing, and the relative expression of ERG11 gene and efflux pump genes CDR1, CDR2 and MDR1, was evaluated by quantitative real-time PCR (q-PCR). Results: No mutations were detected in the ERG11 gene and its overexpression was found only in 12.5% of the resistant strains (1/8). The most prevalent mechanism of resistance was the over-expression of efflux pumps (62.5%; 5/8). Conclusion: The study of the expression of efflux pumps by q-PCR could be a useful diagnostic tool for early detection of azole resistance in C. albicans.Palavras-chave : Candida albicans; resistance; azole; efflux pump; ERG11; CDR1; CDR2; MDR1.
Starting Page	511
Ending Page	517
File Format	HTM / HTML
ISSN	07161018
DOI	10.4067/S0716-10182014000500001
Issue Number	5
Journal	Revista chilena de infectología
Volume Number	31
Language	English Spanish
Publisher Date	2014-01-10
Access Restriction	Open
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Public Health, Environmental and Occupational Health

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