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Feeding by Amblyomma maculatum (Acari: Ixodidae) Enhances Rickettsia parkeri (Rickettsiales: Rickettsiaceae) Infection in the Skin
| Content Provider | Oxford Academic |
|---|---|
| Author | Grasperge, Britton J. Morgan, Timothy W. Paddock, Christopher D. Peterson, Karin E. Macaluso, Kevin R. |
| Copyright Year | 2014 |
| Abstract | Rickettsia parkeri Luckman (Rickettsiales: Rickettsiaceae), a member of the spotted fever group of Rickettsia, is the tick-borne causative agent of a newly recognized, eschar-associated rickettsiosis. Because of its relatively recent designation as a pathogen, few studies have examined the pathogenesis of transmission of R. parkeri to the vertebrate host. To further elucidate the role of tick feeding in rickettsial infection of vertebrates, nymphal Amblyomma maculatum Koch (Acari: Ixodidae) were fed on C3H/HeJ mice intradermally inoculated with R. parkeri (Portsmouth strain). The ticks were allowed to feed to repletion, at which time samples were taken for histopathology, immunohistochemistry (IHC), quantitative polymerase chain reaction (qPCR) for rickettsial quantification, and reverse transcriptase polymerase chain reaction (RT-PCR) for expression of Itgax, Mcp1, and Il1β. The group of mice that received intradermal inoculation of R. parkeri with tick feeding displayed significant increases in rickettsial load and IHC staining, but not in cytokine expression, when compared with the group of mice that received intradermal inoculation of R. parkeri without tick feeding. Tick feeding alone was associated with histopathologic changes in the skin, but these changes, and particularly vascular pathology, were more pronounced in the skin of mice inoculated previously with R. parkeri and followed by tick feeding. The marked differences in IHC staining and qPCR for the R. parkeri with tick feeding group strongly suggest an important role for tick feeding in the early establishment of rickettsial infection in the skin. |
| Related Links | https://academic.oup.com/jme/article-pdf/51/4/855/18248663/jmedent51-0855.pdf |
| Ending Page | 863 |
| Starting Page | 855 |
| File Format | |
| ISSN | 00222585 |
| e-ISSN | 19382928 |
| DOI | 10.1603/ME13248 |
| Journal | Journal of Medical Entomology |
| Issue Number | 4 |
| Volume Number | 51 |
| Language | English |
| Publisher | Oxford Academic |
| Publisher Date | 2014-07-01 |
| Access Restriction | Open |
| Subject Keyword | Biological Sciences Entomology Invertebrates Medicine and Health Public Health and Epidemiology Science and Mathematics Zoology and Animal Sciences Rickettsia Amblyomma Tick-borne C3h/hej |
| Content Type | Text |
| Resource Type | Article |
| Subject | Health Informatics 1100/1109; 3400; 2400/2405; 2700/2725 |
