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The pattern of GPI-80 expression is a useful marker for unusual myeloid maturation in peripheral blood
| Content Provider | Oxford Academic |
|---|---|
| Author | Takeda, Y Kato, T Ito, H Kurota, Y Yamagishi, A Sakurai, T Araki, A Nara, H Tsuchiya, N Asao, H |
| Copyright Year | 2016 |
| Abstract | Myeloid-derived suppressor cells (MDSCs) have a wide spectrum of immunosuppressive activity; control of these cells is a new target for improving clinical outcomes in cancer patients. MDSCs originate from unusual differentiation of neutrophils or monocytes induced by inflammatory cytokines, including granulocyte-colony stimulating factor (G-CSF) and granulocyte–macrophage (GM)-CSF. However, MDSCs are difficult to detect in neutrophil or monocyte populations because they are not uniform cells, resembling both neutrophils and monocytes; thus, they exist in a heterogeneous population. In this study, we investigated GPI-80, a known regulator of Mac-1 (CD11b/CD18) and associated closely with neutrophil maturation, to clarify this unusual differentiation. First, we demonstrated that the mean fluorescence intensity (MFI) of GPI-80 and coefficient of variation (CV) of GPI-80 were increased by treatment with G-CSF and GM-CSF, respectively, using a human promyelocytic leukaemia (HL60) cell differentiation model. To confirm the value of GPI-80 as a marker of unusual differentiation, we measured GPI-80 expression and MDSC functions using peripheral blood cells from metastatic renal cell carcinoma patients. The GPI-80 CV was augmented significantly in the CD16hi neutrophil cell population, and GPI-80 MFI was increased significantly in the CD33hi monocyte cell population. Furthermore, the GPI-80 CV in the CD16hi population was correlated inversely with the proliferative ability of T cells and the GPI-80 MFI of the CD33hi population was correlated with reactive oxygen species production. These results led us to propose that the pattern of GPI-80 expression in these populations is a simple and useful marker for unusual differentiation, which is related to MDSC functions. |
| Related Links | https://academic.oup.com/cei/article-pdf/186/3/373/41642575/cei12859.pdf |
| Ending Page | 386 |
| Starting Page | 373 |
| File Format | |
| ISSN | 00099104 |
| e-ISSN | 13652249 |
| DOI | 10.1111/cei.12859 |
| Journal | Clinical and Experimental Immunology |
| Issue Number | 3 |
| Volume Number | 186 |
| Language | English |
| Publisher | Oxford Academic |
| Publisher Date | 2016-11-09 |
| Access Restriction | Open |
| Subject Keyword | Clinical Medicine Immunology Medicine and Health Pathology Cytokine Granulocyte Colony-stimulating Factor Granulocyte-macrophage Colony-stimulating Factor Recombinant Granulocyte-macrophage Colony-stimulating Factors Monocytes Myeloid Cells Neutrophils Macrophage-1 Antigen T-lymphocytes Antigens, Cd16 Neutrophil Production Cd33 Antigen Myeloid-derived Suppressor Cells Fluorescence Reactive Oxygen Species Heterogeneity Granulocytes Acute Promyelocytic Leukemia Hl-60 Cells Macrophages Cancer Immunosuppressive Agents Renal Cell Carcinoma Metastatic Neutrophilic Cells Treatment Outcome Cell Differentiation Cd18 Antigens Blood Cells Renal Cell Carcinoma Gpi-80/vnn2 Mdscs Myeloid Cell Differentiation |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Immunology |
