Loading...
Please wait, while we are loading the content...
Similar Documents
Gene dosage compensation of rRNA transcript levels in Arabidopsis thaliana lines with reduced ribosomal gene copy number
| Content Provider | Oxford Academic |
|---|---|
| Author | Lopez, Francesca B Fort, Antoine Tadini, Luca Probst, Aline V McHale, Marcus Friel, James Ryder, Peter Pontvianne, Frédéric Pesaresi, Paolo Sulpice, Ronan McKeown, Peter Brychkova, Galina Spillane, Charles |
| Copyright Year | 2021 |
| Abstract | The 45S rRNA genes (rDNA) are among the largest repetitive elements in eukaryotic genomes. rDNA consists of tandem arrays of rRNA genes, many of which are transcriptionally silenced. Silent rDNA repeats may act as 'back-up' copies for ribosome biogenesis and have nuclear organization roles. Through Cas9-mediated genome editing in the Arabidopsis thaliana female gametophyte, we reduced 45S rDNA copy number (CN) to a plateau of ∼10%. Two independent lines had rDNA CNs reduced by up to 90% at the T7 generation, named low copy number (LCN) lines. Despite drastic reduction of rDNA copies, rRNA transcriptional rates, and steady-state levels remained the same as wild-type plants. Gene dosage compensation of rRNA transcript levels was associated with reduction of silencing histone marks at rDNA loci and altered Nucleolar Organiser Region 2 organization. Although overall genome integrity of LCN lines appears unaffected, a chromosome segmental duplication occurred in one of the lines. Transcriptome analysis of LCN seedlings identified several shared dysregulated genes and pathways in both independent lines. Cas9 genome editing of rRNA repeats to generate LCN lines provides a powerful technique to elucidate rDNA dosage compensation mechanisms and impacts of low rDNA CN on genome stability, development, and cellular processes. |
| Related Links | https://academic.oup.com/plcell/article-pdf/33/4/1135/38821967/koab020.pdf |
| Ending Page | 1150 |
| Starting Page | 1135 |
| File Format | |
| ISSN | 10404651 |
| e-ISSN | 1532298X |
| DOI | 10.1093/plcell/koab020 |
| Journal | The Plant Cell |
| Issue Number | 4 |
| Volume Number | 33 |
| Language | English |
| Publisher | Oxford Academic |
| Publisher Date | 2021-05-31 |
| Access Restriction | Open |
| Subject Keyword | Biological Sciences Botany Plant Development Plant Evolution Plant Physiology Plant Reproduction and Propagation Plant Sciences and Forestry Science and Mathematics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Plant Science |
