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Photosynthetic-Product–Dependent Activation of Plasma Membrane H+-ATPase and Nitrate Uptake in Arabidopsis Leaves
| Content Provider | Oxford Academic |
|---|---|
| Author | Kinoshita, Satoru N Suzuki, Takamasa Kiba, Takatoshi Sakakibara, Hitoshi Kinoshita, Toshinori |
| Copyright Year | 2023 |
| Abstract | Plasma membrane (PM) proton-translocating adenosine triphosphatase (H+-ATPase) is a pivotal enzyme for plant growth and development that acts as a primary transporter and is activated by phosphorylation of the penultimate residue, threonine, at the C-terminus. Small Auxin-Up RNA family proteins maintain the phosphorylation level via inhibiting dephosphorylation of the residue by protein phosphatase 2C-D clade. Photosynthetically active radiation activates PM H+-ATPase via phosphorylation in mesophyll cells of Arabidopsis thaliana, and phosphorylation of PM H+-ATPase depends on photosynthesis and photosynthesis-related sugar supplementation, such as sucrose, fructose and glucose. However, the molecular mechanism and physiological role of photosynthesis-dependent PM H+-ATPase activation are still unknown. Analysis using sugar analogs, such as palatinose, turanose and 2-deoxy glucose, revealed that sucrose metabolites and products of glycolysis such as pyruvate induce phosphorylation of PM H+-ATPase. Transcriptome analysis showed that the novel isoform of the Small Auxin-Up RNA genes, SAUR30, is upregulated in a light- and sucrose-dependent manner. Time-course analyses of sucrose supplementation showed that the phosphorylation level of PM H+-ATPase increased within 10 min, but the expression level of SAUR30 increased later than 10 min. The results suggest that two temporal regulations may participate in the regulation of PM H+-ATPase. Interestingly, a 15NO3− uptake assay in leaves showed that light increases 15NO3− uptake and that increment of 15NO3− uptake depends on PM H+-ATPase activity. The results opened the possibility of the physiological role of photosynthesis-dependent PM H+-ATPase activation in the uptake of NO3−. We speculate that PM H+-ATPase may connect photosynthesis and nitrogen metabolism in leaves. |
| Related Links | https://academic.oup.com/pcp/article-pdf/64/2/191/49377911/pcac157.pdf |
| Ending Page | 203 |
| Starting Page | 191 |
| File Format | |
| ISSN | 00320781 |
| e-ISSN | 14719053 |
| DOI | 10.1093/pcp/pcac157 |
| Journal | Plant and Cell Physiology |
| Issue Number | 2 |
| Volume Number | 64 |
| Language | English |
| Publisher | Oxford Academic |
| Publisher Date | 2023-03-01 |
| Access Restriction | Open |
| Subject Keyword | Biological Sciences Molecular and Cell Biology Plant Sciences and Forestry Science and Mathematics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Physiology Plant Science Medicine |
