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TDP-43 real-time quaking induced conversion reaction optimization and detection of seeding activity in CSF of amyotrophic lateral sclerosis and frontotemporal dementia patients
| Content Provider | Oxford Academic |
|---|---|
| Author | Scialò, Carlo Tran, Thanh Hoa Salzano, Giulia Novi, Giovanni Caponnetto, Claudia Chiò, Adriano Calvo, Andrea Canosa, Antonio Moda, Fabio Caroppo, Paola Silani, Vincenzo Ticozzi, Nicola Ratti, Antonia Borroni, Barbara Benussi, Luisa Ghidoni, Roberta Furlanis, Giovanni Manganotti, Paolo Senigagliesi, Beatrice Parisse, Pietro Brasselet, Romain Buratti, Emanuele Legname, Giuseppe |
| Copyright Year | 2020 |
| Abstract | The pathological deposition of the transactive response DNA-binding protein of 43 kDa occurs in the majority (∼97%) of amyotrophic lateral sclerosis and in around 45% of frontotemporal lobar degeneration cases. Amyotrophic lateral sclerosis and frontotemporal lobar degeneration clinically overlap, presenting a continuum of phenotypes. Both amyotrophic lateral sclerosis and frontotemporal lobar degeneration lack treatments capable of interfering with the underlying pathological process and early detection of transactive response DNA-binding protein of 43 kDa pathology would facilitate the development of disease-modifying drugs. The real-time quaking-induced conversion reaction showed the ability to detect prions in several peripheral tissues of patients with different forms of prion and prion-like diseases. Despite transactive response DNA-binding protein of 43 kDa displays prion-like properties, to date the real-time quaking-induced conversion reaction technology has not yet been adapted to this protein. The aim of this study was to adapt the real-time quaking-induced conversion reaction technique for the transactive response DNA-binding protein of 43 kDa substrate and to exploit the intrinsic ability of this technology to amplify minute amount of mis-folded proteins for the detection of pathological transactive response DNA-binding protein of 43 kDa species in the cerebrospinal fluid of amyotrophic lateral sclerosis and frontotemporal lobar degeneration patients. We first optimized the technique with synthetic transactive response DNA-binding protein of 43 kDa–pre-formed aggregates and with autopsy-verified brain homogenate samples and subsequently analysed CSF samples from amyotrophic lateral sclerosis and frontotemporal lobar degeneration patients and controls. Transactive response DNA-binding protein of 43 kDa real-time quaking-induced conversion reaction was able to detect as little as 15 pg of transactive response DNA-binding protein of 43 kDa aggregates, discriminating between a cohort of patients affected by amyotrophic lateral sclerosis and frontotemporal lobar degeneration and age-matched controls with a total sensitivity of 94% and a specificity of 85%. Our data give a proof-of-concept that transactive response DNA-binding protein of 43 kDa is a suitable substrate for the real-time quaking-induced conversion reaction. Transactive response DNA-binding protein of 43 kDa real-time quaking-induced conversion reaction could be an innovative and useful tool for diagnosis and drug development in amyotrophic lateral sclerosis and frontotemporal lobar degeneration. The cerebrospinal fluid detection of transactive response DNA-binding protein of 43 kDa pathological aggregates may be exploited as a disease biomarker for amyotrophic lateral sclerosis and frontotemporal lobar degeneration patients. |
| Related Links | https://academic.oup.com/braincomms/article-pdf/2/2/fcaa142/33892798/fcaa142.pdf |
| File Format | |
| e-ISSN | 26321297 |
| DOI | 10.1093/braincomms/fcaa142 |
| Journal | Brain Communications |
| Issue Number | 2 |
| Volume Number | 2 |
| Language | English |
| Publisher | Oxford Academic |
| Publisher Date | 2020-07-01 |
| Access Restriction | Open |
| Subject Keyword | Clinical Medicine Medicine and Health Neurology Neuroscience Science and Mathematics Amyotrophic Lateral Sclerosis Protein Tdp-43 Real-time Quaking-induced Conversion Frontotemporal Dementia Frontotemporal Lobar Degeneration Tdp-43 Rt-quic Als Ftd Biomarker |
| Content Type | Text |
| Resource Type | Article |
| Subject | Psychiatry and Mental Health Neurology Cellular and Molecular Neuroscience Biological Psychiatry |
