Loading...
Please wait, while we are loading the content...
Similar Documents
Quantitative Analysis of Tetramethylenedisulfotetramine ("Tetramine") Spiked into Beverages by Liquid Chromatography Tandem Mass Spectrometry with Validation by Gas Chromatography Mass Spectrometry
| Content Provider | United States Department of Energy Office of Scientific and Technical Information (OSTI.GOV) |
|---|---|
| Author | Owens, J. Hok, S. Alcaraz, A. |
| Organization | Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States) |
| Abstract | Tetramethylenedisulfotetramine, commonly known as tetramine, is a highly neurotoxic rodenticide (human oral LD{sub 50} = 0.1 mg/kg) used in hundreds of deliberate food poisoning events in China. Here we describe a method for quantitation of tetramine spiked into beverages, including milk, juice, tea, cola, and water and cleaned up by C8 solid phase extraction and liquid-liquid extraction. Quantitation by high performance liquid chromatography tandem mass spectrometry (LC/MS/MS) was based upon fragmentation of m/z 347 to m/z 268. The method was validated by gas chromatography mass spectrometry (GC/MS) operated in SIM mode for ions m/z 212, 240, and 360. The limit of quantitation was 0.10 {micro}g/mL by LC/MS/MS versus 0.15 {micro}g/mL for GC/MS. Fortifications of the beverages at 2.5 {micro}g/mL and 0.25 {micro}g/mL were recovered ranging from 73-128% by liquid-liquid extraction for GC/MS analysis, 13-96% by SPE and 10-101% by liquid-liquid extraction for LC/MS/MS analysis. |
| Sponsorship | USDOE |
| Related Links | https://www.osti.gov/biblio/957169 |
| Page Count | 34 |
| File Size | 1048576 |
| File Format | |
| ISSN | 00218561 |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume Number | 57 |
| Issue Number | 10 |
| Language | English |
| Publisher Date | 2008-11-13 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY BEVERAGES CHROMATOGRAPHY FOOD FRAGMENTATION GAS CHROMATOGRAPHY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROSCOPY MILK POISONING SOLAR PROTONS SOLVENT EXTRACTION VALIDATION |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Agricultural and Biological Sciences |
