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Stimuli Responsive Hierarchical Assembly of P22 Virus-like Particles
| Content Provider | United States Department of Energy Office of Scientific and Technical Information (OSTI.GOV) |
|---|---|
| Author | Aumiller, William M. (Jr.) Uchida, Masaki Biner, Daniel W. Miettinen, Heini M. Lee, Byeongdu Douglas, Trevor |
| Organization | Argonne National Lab. (ANL), Argonne, IL (United States) |
| Abstract | Biomimetic systems responsive to environmental stimuli are of growing interest due to their useful, intriguing, and sometimes unexpected properties. Hierarchical self-assembly of biological building blocks has emerged as a powerful means of creating biomaterials with collective properties. Here, we show P22 virus-like particles (VLPs) functionalized with a spider silk protein derivative on the capsid exterior assemble into a hierarchical structure due to spider silk/spider silk interactions at low pH and reversibly dissemble upon raising the pH. We also show that the capsid arrays can be assembled through electrostatic interaction between the negatively charged capsids and a positively supercharged GFP mutant and can be reversed by raising the ionic strength. Most notably, we found that the supercharged GFP could bind to the hierarchically assembled material under high salt conditions but not to the individual capsids under the same high salt conditions. As a result, the binding of the charged macromolecule under high salt conditions demonstrates a collective behavior of the hierarchically assembled system that is not present with the unassembled, individual components of the system. |
| Sponsorship | USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22) |
| Related Links | https://www.osti.gov/biblio/1461344 |
| Starting Page | 2262 |
| Ending Page | 2273 |
| Page Count | 12 |
| File Format | |
| ISSN | 08974756 |
| DOI | 10.1021/acs.chemmater.7b04964 |
| Journal | Chemistry of Materials |
| Volume Number | 30 |
| Issue Number | 7 |
| Language | English |
| Publisher | American Chemical Society (ACS) |
| Publisher Date | 2018-03-15 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Materials Chemistry Chemical Engineering |
