NDLI: Test No. 241: The Larval Amphibian Growth and Development Assay (LAGDA)

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Test No. 218: Sediment-Water Chironomid Toxicity Using Spiked Sediment

Test No. 219: Sediment-Water Chironomid Toxicity Using Spiked Water

Test No. 220: Enchytraeid Reproduction Test

Test No. 222: Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei)

Test No. 215: Fish, Juvenile Growth Test

Test No. 216: Soil Microorganisms: Nitrogen Transformation Test

Test No. 217: Soil Microorganisms: Carbon Transformation Test

Test No. 212: Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages

Test No. 213: Honeybees, Acute Oral Toxicity Test

Test No. 214: Honeybees, Acute Contact Toxicity Test

Test No. 203: Fish, Acute Toxicity Test

Test No. 204: Fish, Prolonged Toxicity Test: 14-Day Study

Test No. 205: Avian Dietary Toxicity Test

Test No. 206: Avian Reproduction Test

Test No. 207: Earthworm, Acute Toxicity Tests

Test No. 241: The Larval Amphibian Growth and Development Assay (LAGDA)

Content Provider	OECD iLibrary
Organization	OECD
Abstract	The test guideline of the Larval Amphibian Growth and Development Assay (LAGDA) describes a toxicity test with an amphibian species (African clawed frog ( Xenopus laevis )) that considers growth and development from fertilization through the early juvenile period. It is an assay (typically 16 weeks) that assesses early development, metamorphosis, survival, growth, and partial reproductive maturation. It also enables measurement of a suite of other endpoints that allows for diagnostic evaluation of suspected endocrine disrupting chemicals (EDCs) or other types of developmental and reproductive toxicants. The LAGDA serves as a higher tier test with an amphibian for collecting more comprehensive concentration-response information on adverse effects suitable for use in hazard identification and characterization, and in ecological risk assessment. The general experimental design entails exposing X. laevis embryos at Nieuwkoop and Faber (NF) stage 8-10 (3) to a minimum of four different concentrations of test chemical and control(s) until 10 weeks after the median time to NF stage 62. There are four replicates in each test concentration with eight replicates for the control. Endpoints evaluated during the course of the exposure (at the interim sub-sample and final sample at completion of the test) include those indicative of generalized toxicity: mortality, abnormal behaviour, and growth determinations (length and weight), as well as endpoints designed to characterize specific endocrine toxicity modes of action targeting oestrogen, androgen or thyroid-mediated physiological processes.
Page Count	38
ISSN	20745761
e-ISBN	9789264242340
Language	English
Publisher	OECD Publishing
Publisher Date	2015-07-28
Access Restriction	Open
Subject Keyword	Environment
Content Type	Text
Resource Type	Book

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Test No. 229: Fish Short Term Reproduction Assay

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Test No. 215: Fish, Juvenile Growth Test

Test No. 241: The Larval Amphibian Growth and Development Assay (LAGDA)

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