NDLI: L-myo-InositoL-1- phosphate synthase from bryophytes: purification and characterization of the enzyme from Lunularia cruciata (L.) Dum

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L-myo-InositoL-1- phosphate synthase from bryophytes: purification and characterization of the enzyme from Lunularia cruciata (L.) Dum

Content Provider	Open Access Library (OALib)
Author	Chhetri Dhani Raj Yonzone Sachina Mukherjee Asok Kumar Tamang Sanjeeta
Abstract	l-myo-inositol-1-phosphate synthase (mips; ec: 5.5.1.4) catalyzes the conversion of d-glucose-6-phosphate to 1l-myo-inositol-1-phosphate, the rate limiting step in the biosynthesis of all inositol containing compounds. myo-inositol and its derivatives are implicated in membrane biogenesis, cell signaling, salinity stress tolerance and a number of other metabolic reactions in different organisms. this enzyme has been reported from a number of bacteria, fungi, plants and animals. in the present study some bryophytes available in the eastern himalaya have been screened for free myo-inositol content. it is seen that bryum corinatum, a bryopsid shows the highest content of free myo-inositol among the species screened. subsequently , the enzyme mips has been partially purified to the tune of about 70 fold with approximately 18% recovery form the reproductive part bearing gametophytes of lunularia cruciata. the l. cruciata synthase specifically utilized d-glucose-6-phosphate and nad+ as its substrate and co-factor respectively. the optimum ph shown was 7.0 while the temperature maximum was at 30oc. the enzyme activity was slightly stimulated by mg2+ and ca2+; remarkably stimulated by nh4+; slightly inhibited by mn2+; highly inhibited by cu2+, zn2+ and hg2+. the km values for d-glucose-6-phosphate and nad+ was found to be 0.80 and 0. 034 mm respectively while the vmax values were 2.8 and 1.21 mm for d-glucose-6-phosphate and nad+ respectively.
ISSN	16770420
Journal	Brazilian Journal of Plant Physiology
DOI	10.1590/S1677-04202009000300008
Publisher	Scientific Electronic Library Online
Publisher Date	2009-01-01
Access Restriction	Open
Subject Keyword	Inositol monophosphatase D-glucose-6-phosphate Inositol synthase L-myo-inositol-1-phosphate. Myo-inositol
Content Type	Text
Resource Type	Article
Subject	Plant Science Agronomy and Crop Science

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