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Serodiagnosis of tuberculosis: specific detection of free and complex-dissociated antibodies anti-mycobacterium tuberculosis recombinant antigens
| Content Provider | Open Access Library (OALib) |
|---|---|
| Author | Schmelling María Fernanda Imaz María Susana Singh Mahavir Spallek Ralph Kaempfer Susanne |
| Abstract | the diagnostic test characteristics of detecting free and complex-dissociated igg to three recombinant antigens of mycobacterium tuberculosis (38-kda, ag16 and ag85b), singly and in combination, were evaluated in sera from 161 tuberculous patients [smear-positive pulmonary tb (50), smear-negative pulmonary tb (ptbsm-) (60) and extrapulmonary tb (51)) and 214 control patients (mycobacteriosis (14), mycoses(14), leprosy(4), other underlying diseases (82) and healthy people (100)]. the individual antigens ranged from 25% to 42% in sensitivity and from 93% to 96% in specificity, while considering free igg response. addition of complex-dissociated antibodies against each individual antigen improved the sensitivity up to 55%. the number and levels of specific antibodies varied greatly from individual to individual. combination of individual results for free and complex-dissociated igg to 38-kda, ag16 and ag85b offered 76% sensitivity and 83% specificity. when the three antigens were placed in the same well, the sensitivity was lower than that expected on the basis of single antigen (63%) but with a good specificity (95%), even in the group of mycobacteriosis or mycoses. the highest contribution of complex-dissociated igg results to free igg results was seen for the diagnosis of ptbsm- patients. in conclusion, although neither single recombinant antigen was reactive with most sera from tb patients even after the measurement of both free and complex-dissociated antibodies, the use of multi-antigen cocktails improved the diagnostic utility of the elisa assay, allowing the identification of almost 70% of ptbsm-, with a high level of specificity; the use of additional, well selected antigens should lead to the detection of almost all patients with tb. |
| ISSN | 14138670 |
| Journal | Brazilian Journal of Infectious Diseases |
| DOI | 10.1590/S1413-86702008000300014 |
| Publisher | Brazilian Society of Infectious Diseases |
| Publisher Date | 2008-01-01 |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
| Subject | Infectious Diseases Microbiology (medical) |
