NDLI: Nuclease p1 immobilized on deae cellulose

Content Provider	Open Access Library (OALib)
Author	Yi Yu Xiong Wen-Yue Tang Zhen-Xing Ying Guo-Qing Mei Jiang-Feng Shi Lu-E
Abstract	effects of various factors, such as ph, ionic strength, glutaraldehyde concentration, enzyme amount and immobilization time, on enzyme activity were investigated. the immobilization conditions were optimized by orthogonal experiments. characterizations of immobilized nuclease p1 were also evaluated. through orthogonal optimization, the optimal immobilization conditions were as follows: ph 5.6, ionic strength 0.125, glutaraldehyde concentration 0.20% and immobilization time 2.0 h. optimal ph of immobilized enzyme was 5.8. optimal temperature of immobilized enzyme was 70oc. thermal, operational and storage stabilities of the enzyme were improved after it was immobilized on deae cellulose. michaelis constant km of immobilized enzyme at 69oc was found to be 27.21 g/l by the lineweaver-burk plot.
ISSN	01046632
Journal	Brazilian Journal of Chemical Engineering
DOI	10.1590/S0104-66322010000100003
Publisher	Brazilian Society of Chemical Engineering
Publisher Date	2010-01-01
Access Restriction	Open
Subject Keyword	Immobilization Characterization. Nuclease p1 Deae cellulose
Content Type	Text
Resource Type	Article
Subject	Chemical Engineering

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in