Loading...
Please wait, while we are loading the content...
Similar Documents
Evolution, expression differentiation and interaction specificity of heterotrimeric G-protein subunit gene family in the mesohexaploid Brassica rapa
| Content Provider | National Institute of Plant Genome Research (NIPGR) |
|---|---|
| Author | Arya, Gulab C. Kumar, Roshan Bisht, Naveen C. |
| Abstract | Heterotrimeric G-proteins, comprising of Gα, Gβ, and Gγ subunits, are important signal transducers which regulate many aspects of fundamental growth and developmental processes in all eukaryotes. Initial studies in model plants Arabidopsis and rice suggest that the repertoire of plant G-protein is much simpler than that observed in metazoans. In order to assess the consequence of whole genome triplication events within Brassicaceae family, we investigated the multiplicity of G-protein subunit genes in mesohexaploid Brassica rapa, a globally important vegetable and oilseed crop. We identified one Gα (BraA.Gα1), three Gβ (BraA.Gβ1, BraA.Gβ2, and BraA.Gβ3), and five Gγ (BraA.Gγ1, BraA.Gγ2, BraA.Gγ3, BraA.Gγ4, and BraA.Gγ5) genes from B. rapa, with a possibility of 15 Gαβγ heterotrimer combinations. Our analysis suggested that the process of genome triplication coupled with gene-loss (gene-fractionation) phenomenon have shaped the quantitative and sequence diversity of G-protein subunit genes in the extant B. rapa genome. Detailed expression analysis using qRT-PCR assays revealed that the G-protein genes have retained ubiquitous but distinct expression profiles across plant development. The expression of multiple G-protein genes was differentially regulated during seed-maturation and germination stages, and in response to various phytohormone treatments and stress conditions. Yeast-based interaction analysis showed that G-protein subunits interacted in most of the possible combinations, with some degree of subunit-specific interaction specificity, to control the functional selectivity of G-protein heterotrimer in different cell and tissue-types or in response to different environmental conditions. Taken together, this research identifies a highly diverse G-protein signaling network known to date from B. rapa, and provides a clue about the possible complexity of G-protein signaling networks present across globally important Brassica species. |
| Sponsorship | This work was supported by the core research grant from National Institute of Plant Genome Research, India. GCA and RK were supported by a Junior Research Fellowship from Council of Scientific and Industrial Research (CSIR, India) and University Grant Commission (UGC, India), respectively. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
| File Format | |
| ISSN | 19326203 |
| Journal | PLoS One |
| Volume Number | 9 |
| Issue Number | 9 |
| DOI | 10.1371/journal.pone.0105771 |
| Language | English |
| Publisher | PLOS |
| Publisher Date | 2014-07-28 |
| Access Restriction | Open |
| Subject Keyword | G-Protein Heterotrimeric G-proteins Brassica rapa |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
