NDLI: ISSR marker assisted selection of male and female plants in a promising dioecious crop-Jojoba (Simmondsia chinensis)

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ISSR marker assisted selection of male and female plants in a promising dioecious crop-Jojoba (Simmondsia chinensis)

Content Provider	National Institute of Plant Genome Research (NIPGR)
Author	Sharma, Kuldeep Agrawal, Veena Gupta, Sarika Kumar, Ravindra Prasad, Manoj
Abstract	Simmondsia chinensis (Link) Schneider, a multipurpose and monogeneric dioecious shrub from arid zones, has emerged as a cash crop all over the globe. Its seed propagation poses severe problems due to its male-biased population: the male:female ratio is 5:1. Investigations have been carried out to generate a sex-specific Inter-simple sequence repeat (ISSR) marker for the early detection of male and female plants. Of the 42 primers analysed with a bulk sample of pooled male DNA and a bulk sample of pooled female DNA, only one primer, UBC-807, produced a unique ~1,200 base-pair fragment in the male DNA. To validate this observation, this primer was re-tested with individual male and female samples from eight cultivars. A similar unique ~1,200 bp fragment was present in the male individuals of all eight cultivars and completely absent in the female individuals tested. This is the first report of the use of ISSR markers to ascertain sex in physiologically mature S. chinensis plants.
Sponsorship	Veena Agrawal is grateful to the University Grants Commission and the University of Delhi for financial assistance. Facilities provided by the Head of the Department of Botany at the University of Delhi and the Director of the National Institute for Plant Genome Research New Delhi are gratefully acknowledged. We also thank the AJORP (Association of the Rajasthan Jojoba Plantation and Research Project) for research material. Kuldeep Sharma is indebted to CSIR New Delhi for awarding SRF.
Starting Page	239
Ending Page	243
Page Count	5
File Format	PDF
Journal	Plant Biotechnology Reports
Volume Number	2
Language	English
Publisher	Springer
Publisher Date	2008-01-01
Access Restriction	Authorized
Subject Keyword	Dioecious ISSR marker Simmondsia chinensis
Content Type	Text
Resource Type	Article

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