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Transcription factor repertoire of necrotrophic fungal phytopathogen Ascochyta rabiei: predominance of MYB transcription factors as potential regulators of secretome
| Content Provider | National Institute of Plant Genome Research (NIPGR) |
|---|---|
| Author | Verma, Sandhya Gazara, Rajesh Kumar Verma, Praveen Kumar |
| Abstract | Transcription factors (TFs) are the key players in gene expression and their study is highly significant for shedding light on the molecular mechanisms and evolutionary history of organisms. During host pathogen interaction, extensive reprogramming of gene expression facilitated by TFs is likely to occur in both host and pathogen. To date, the knowledge about TF repertoire in filamentous fungi is in infancy. Thenecrotrophic fungus Ascochyta rabiei, that causes destructive Ascochyta blight (AB)disease of chickpea (Cicer arietinum), demands more comprehensive study for better understanding of Ascochyta-legume path system. In the present study, we performed the genome-wide identification and analysis of TFs in A. rabiei. Taking advantage ofA. rabiei genome sequence, we used a bioinformatics approach to predict the TFrepertoire of A. rabiei. For identification and classification of A. rabiei TFs, we designed comprehensive pipeline using a combination of BLAST and InterProScan software. A total of 381 A. rabiei TFs were predicted and divided into 32 fungal specific families ofTFs. The gene structure, domain organization and phylogenetic analysis of abundant families of A. rabiei TFs were also carried out. Comparative study of A. rabiei TFswith that of other necrotrophic, biotrophic, hemibiotrophic, symbiotic, and saprotrophicfungi was performed. It suggested presence of both conserved as well as unique features among them. Moreover, cis-acting elements on promoter sequences of earlier predicted A. rabiei secretome were also identified. With the help of published A. rabieitranscriptome data, the differential expression of TF and secretary protein coding geneswas analyzed. Furthermore, comprehensive expression analysis of few selected A. rabieiTFs using quantitative real-time polymerase chain reaction revealed variety of expression patterns during host colonization. These genes were expressed in at least one of the time points tested post infection. Overall, this study illustrates the first genome-wide identification and analysis of TF repertoire of A. rabiei. This work would provide the basis for further studies to dissect role of TFs in the molecular mechanisms during A. rabieichickpea interactions. |
| Sponsorship | This study was supported by research grant (BT/PR7164/PBD/16/1016/2012) of Department of Biotechnology, Government ofIndia and core grant from National Institute of Plant GenomeResearch, New Delhi, India. |
| ISSN | 1664462X |
| Journal | Frontiers in Plant Science |
| Volume Number | 8 |
| Language | English |
| Publisher | Frontiers Media S.A. |
| Access Restriction | Open |
| Subject Keyword | Plant–pathogen interaction Necrotrophic fungus Cis-acting elements |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science |
