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Improved method of RNA isolation from laser capture microdissection (LCM)-derived plant tissues
| Content Provider | National Institute of Plant Genome Research (NIPGR) |
|---|---|
| Author | Gautam, Vibhav Singh, Archita Singh, Sharmila Verma, Swati Sarkar, Ananda K. |
| Abstract | Laser capture micro dissection (LCM) is a tool to isolate desired and/or less accessible cells or tissues from a heterogeneous population. In the current method, we describe an efficient and cost-effective method to obtain both high-quality mRNA and miRNAs in sufficient quantity from LCM-derived plant tissues. The quality of the isolated RNA can be assessed using Bio analyzer. Using modified stem-loop RT-PCR, we confirmed the presence of 2124 nucleotide (nt) long mature miRNAs. This modified LCM-based method has been found to be suitable for the tissue-specific expression analysis of both genes and small RNAs (miRNAs). |
| Sponsorship | V.G., A.S., and S.S. thank the Council for Scientific and Industrial Research (CSIR), India, and the National Institute of Plant Genome Research (NIPGR), New Delhi, India, for funding and internal grants. V.G. and A.S. also acknowledge the Department of Biotechnology (DBT), India, for fellowship. S.V. thanks the Department of Science and Technology-Science and Engineering Research Board (DST-SERB), India, for National Post Doctoral Fellowship (N-PDF). A.K.S. thanks NIPGR and DBT (Project Grant No. BT/PR12766/BPA/118/63/2015), New Delhi, India, for fellowship and grants. The authors declare no conflict of interests. |
| Starting Page | 89 |
| Ending Page | 98 |
| Page Count | 10 |
| ISBN | 9781493990450 |
| ISSN | 10643745 19406029 |
| Volume Number | 1933 |
| Language | English |
| Publisher | Springer Nature |
| Access Restriction | Limited |
| Subject Keyword | Laser microdissection Gene expression MiRNA Root meristem |
| Content Type | Text |
| Resource Type | Chapter |
| Subject | Genetics Molecular Biology |
