NDLI: Generation and Evaluation of Recombinant Thermostable Newcastle Disease Virus Expressing the HA of H9N2 Avian Influenza Virus

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Generation and Evaluation of Recombinant Thermostable Newcastle Disease Virus Expressing the HA of H9N2 Avian Influenza Virus

Content Provider	MDPI
Author	Zhang, Xiaorong Bo, Zongyi Meng, Chenchen Chen, Yin Zhang, Chengcheng Cao, Yongzhong Wu, Yantao
Copyright Year	2021
Description	H9N2 avian influenza virus (AIV) has become endemic in many countries, causing great economic losses when co-infected with other pathogens. So far, several live vaccines based on Newcastle disease virus (NDV) vectors expressing influenza hemagglutinin (HA) have been developed. However, the thermostable recombinant NDV is rarely reported. In this study, using a thermostable NDV rAHR09 strain as the vector, three recombinant NDVs expressing native HA, chimeric HA ectodomain with transmembrane domain/C-terminal cytoplasmic tail domain from fusion protein of NDV, and HA ectodomain were generated, designated rAHR09-HA, rAHR09-HAF, and rAHR09-HAE. The MDT value of three recombinant NDVs was above 120 h, their ICPI value was about 0.03, and the recombinant NDVs were still infectious when treated for 100 min under 56 °C, which demonstrated that the recombinant NDVs kept the lentogenic and thermostable nature of rAHR09. The immunization data showed that rAHR09-HA and rAHR09-HAF induced a higher HI antibody titer against H9N2 AIV and NDV. After being challenged with H9N2 AIV, the rAHR09-HA and rAHR09-HAF could significantly reduce the virus shedding in cloacal and tracheal swab samples. Our results suggest that rAHR09-HA and rAHR09-HAF might be vaccine candidates against H9N2 AIV.
Starting Page	1606
e-ISSN	19994915
DOI	10.3390/v13081606
Journal	Viruses
Issue Number	8
Volume Number	13
Language	English
Publisher	MDPI
Publisher Date	2021-08-13
Access Restriction	Open
Subject Keyword	Viruses Virology Newcastle Disease Virus Thermostability H9n2 Avian Influenza Virus Ha Protein Transmembrane Domain Recombinant Virus
Content Type	Text
Resource Type	Article

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