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Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors
| Content Provider | MDPI |
|---|---|
| Author | Dijana, Krstić-Milošević Vinterhalter, Branka Banjac, Nevena Vinterhalter, Dragan |
| Copyright Year | 2021 |
| Description | The hairy root clones of Gentiana dinarica cl-B, cl-D, cl-3, and cl-14 were cultivated in parallel in diverse simple bioreactors, including temporary immersion systems $RITA^{®}$ (TIS $RITA^{®}$), bubble column bioreactors (BCB), and Erlenmeyer flasks (EF), and evaluated for biomass production and xanthone content. The obtained results showed that TIS $RITA^{®}$ and BCB containing ½ MS medium with 4% sucrose provided equally good growth conditions in which the majority of the clones displayed the higher percentage of dry matter (DM%), and xanthones norswertianin-1-O-primeveroside (nor-1-O-prim) and norswertianin production than those cultivated in EF. Thin and well branched hairy root clone cl-B grown in BCB for 7 weeks was superior regarding all growth parameters tested, including growth index (19.97), dry weight (2.88 g), and DM% (25.70%) compared to all other clones. Cl-B cultured in TIS $RITA^{®}$ contained the highest amount of nor-1-O-prim (56.82 mg per vessel). In BCB with constant aeration, cl-B accumulated the highest norswertianin content reaching 18.08 mg/vessel. The optimized conditions for cultivation of selected G. dinarica hairy root clones in highly aerated TIS $RITA^{®}$ and BCB systems contribute to the development of bioreactor technology designed for the large scale commercial production of xanthones nor-1-O-prim and norswertianin. |
| Starting Page | 1610 |
| e-ISSN | 22237747 |
| DOI | 10.3390/plants10081610 |
| Journal | Plants |
| Issue Number | 8 |
| Volume Number | 10 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-08-05 |
| Access Restriction | Open |
| Subject Keyword | Plants Horticulture Transformed Roots Bubble Bioreactor Tis Rita® Norswertianin Sucrose |
| Content Type | Text |
| Resource Type | Article |
