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Effects on Lignin Redistribution in Eucalyptus globulus Fibres Pre-Treated by Steam Explosion: A Microscale Study to Cellulose Accessibility
| Content Provider | MDPI |
|---|---|
| Author | Patricia, Castaño-Rivera Regis, Teixeira Mendonça Troncoso-Ortega, Eduardo Castillo, Rosario Reyes-Contreras, Pablo Schiappacasse, Nicolás Parra, Carolina |
| Copyright Year | 2021 |
| Description | The objective of this study was to investigate structural changes and lignin redistribution in Eucalyptus globulus pre-treated by steam explosion under different degrees of severity (S0), in order to evaluate their effect on cellulose accessibility by enzymatic hydrolysis. Approximately 87.7% to 98.5% of original glucans were retained in the pre-treated material. Glucose yields after the enzymatic hydrolysis of pre-treated material improved from 19.4% to 85.1% when S0 was increased from 8.53 to 10.42. One of the main reasons for the increase in glucose yield was the redistribution of lignin as micro-particles were deposited on the surface and interior of the fibre cell wall. This information was confirmed by laser scanning confocal fluorescence and FT-IR imaging; these microscopic techniques show changes in the physical and chemical characteristics of pre-treated fibres. In addition, the results allowed the construction of an explanatory model for microscale understanding of the enzymatic accessibility mechanism in the pre-treated lignocellulose. |
| Starting Page | 507 |
| e-ISSN | 2218273X |
| DOI | 10.3390/biom11040507 |
| Journal | Biomolecules |
| Issue Number | 4 |
| Volume Number | 11 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-03-29 |
| Access Restriction | Open |
| Subject Keyword | Biomolecules Paper and Wood Steam Explosion Eucalyptus Globulus Lignin Droplets Enzymatic Hydrolysis Ft-ir Imaging Chemometric Analysis |
| Content Type | Text |
| Resource Type | Article |
