Loading...
Please wait, while we are loading the content...
Similar Documents
Effect of Methionine Supplementation on Rumen Microbiota, Fermentation, and Amino Acid Metabolism in In Vitro Cultures Containing Nitrate
| Content Provider | MDPI |
|---|---|
| Author | Hassan, Faiz-Ul Guo, Yanxia Li, Mengwei Tang, Zhenhua Peng, Lijuan Liang, Xin Yang, Chengjian |
| Copyright Year | 2021 |
| Description | This study evaluated the effect of methionine on in vitro methane $(CH_{4}$) production, rumen fermentation, amino acid (AA) metabolism, and rumen microbiota in a low protein diet. We evaluated three levels of methionine (M0, 0%; M1, 0.28%; and M2, 1.12%) of in the presence of sodium nitrate (1%) in a diet containing elephant grass (90%) and concentrate (10%). We used an in vitro batch culture technique by using rumen fluid from cannulated buffaloes. Total gas and $CH_{4}$ production were measured in each fermentation bottle at 3, 6, 9, 12, 24, 48, 72 h of incubation. Results revealed that M0 decreased (p < 0.001) the total gas and $CH_{4}$ production, but methionine exhibited no effect on these parameters. M0 decreased (p < 0.05) the individual and total volatile fatty acids (VFAs), while increasing (p < 0.05) the ruminal pH, acetate to propionate ratio, and microbial protein content. Methionine did not affect ruminal AA contents except asparagine, which substantially increased (p = 0.003). M2 increased the protozoa counts, but both M0 and M1 decreased (p < 0.05) the relative abundance of Firmicutes while increasing (p < 0.05) the Campilobacterota and Proteobacteria. However, Prevotella and γ-Proteobacteria were identified as biomarkers in the nitrate group. Our findings indicate that methionine can increase ruminal asparagine content and the population of Compylobactor. |
| Starting Page | 1717 |
| e-ISSN | 20762607 |
| DOI | 10.3390/microorganisms9081717 |
| Journal | Microorganisms |
| Issue Number | 8 |
| Volume Number | 9 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-08-12 |
| Access Restriction | Open |
| Subject Keyword | Microorganisms Agriculture and Animal Production Methionine Rumen Microbiota Methanogenesis Amino Acid Metabolism Buffalo |
| Content Type | Text |
| Resource Type | Article |
