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Composition and Antioxidant Activity, Supercritical Carbon Dioxide Extraction Extracts, and Residue after Extraction of Biologically Active Compounds from Freeze-Dried Tomato Matrix
| Content Provider | MDPI |
|---|---|
| Author | Dalia, Urbonavičienė Ramun, ė Bobinaitė Lina, Raudonė Sonata, Trumbeckaitė Jonas, Viškelis Pranas, Viškelis Bobinas, Česlovas |
| Copyright Year | 2021 |
| Description | Supercritical carbon dioxide extraction (SCE-CO2) is an attractive, green technology that is used for the recovery of biologically active compounds from plant material. The antioxidant potential of lipophilic fractions (extract obtained with SCE-CO2) and hydrophilic fractions (extracts obtained from the residue after extraction) obtained from a matrix of freeze-dried tomatoes (cvs. “Admiro” F1, “Jurgiai”, “Vilina”, “Pirmutis”, and “Skariai”) was assessed via different antioxidant activity methods. The total amount of polyphenols, carotenoids, and carotenoid isomers before and after SCE-CO2 extraction was also determined. To investigate the effect of the SCE-CO2 extract on the viability of cancer cells, rat glioblastoma C6 cells were chosen. The SCE-CO2 yielded an average of 800 mg of lipophilic fraction per 100 g of freeze-dried tomatoes. The ABTS•+ scavenging activity of the extract was 251 ± 3.4 µmol TE/g. After SCE-CO2 extraction, the DPPH•-RSA of the freeze-dried tomato matrix was 7 to 12% higher. There was a strong positive correlation (R = 0.84) between the total polyphenolics content and the DPPH•-RSA of the tomato samples. The SCE-CO2 increased the radical scavenging activity of the extraction residue, indicating that a considerable fraction of the hydrophilic compounds with particular antioxidant capacity remain unextracted from the tomato matrix. Our results reveal the cytotoxic effect of lycopene extract rich in cis-isomers (62% cis-isomers of the total lycopene content) on rat glioblastoma C6 cells. The viability of the glioblastoma C6 cells significantly decreased (−42%) at a total lycopene concentration of 2.4 µM after 24 h of incubation. |
| Starting Page | 467 |
| e-ISSN | 22279717 |
| DOI | 10.3390/pr9030467 |
| Journal | Processes |
| Issue Number | 3 |
| Volume Number | 9 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-03-05 |
| Access Restriction | Open |
| Subject Keyword | Processes Integrative and Complementary Medicine Lycopene Tomato Supercritical Fluid Extracts Antioxidant Activity Cytotoxic Effect |
| Content Type | Text |
| Resource Type | Article |
