Loading...
Please wait, while we are loading the content...
Similar Documents
Corn Cob as a Green Support for Laccase Immobilization—Application on Decolorization of Remazol Brilliant Blue R
| Content Provider | MDPI |
|---|---|
| Author | Santos, Priscila M. dos Baruque, Julia R. de Souza Lira, Regiane K. Leite, Selma G. F. Nascimento, Rodrigo P. Do Borges, Cristiano P. Wojcieszak, Robert Itabaiana, Ivaldo |
| Copyright Year | 2022 |
| Description | The high demand for food and energy imposed by the increased life expectancy of the population has driven agricultural activity, which is reflected in the larger quantities of agro-industrial waste generated, and requires new forms of use. Brazil has the greatest biodiversity in the world, where corn is one of the main agricultural genres, and where over 40% of the waste generated is from cobs without an efficient destination. With the aim of the valorization of these residues, we proposed to study the immobilization of laccase from Aspergillus spp. $(L_{Asp}$) in residual corn cob and its application in the degradation of Remazol Brilliant Blue R (RBBR) dye. The highest yields in immobilized protein (75%) and residual activity (40%) were obtained at pH 7.0 and an enzyme concentration of 0.1 $g.mL^{−1}$, whose expressed enzyme activity was 1854 $U.kg^{−1}$. At a temperature of 60 °C, more than 90% of the initial activity present in the immobilized biocatalyst was maintained. The immobilized enzyme showed higher efficiency in the degradation (64%) of RBBR dye in 48 h, with improvement in the process in 72 h (75%). The new biocatalyst showed operational efficiency during three cycles, and a higher degradation rate than the free enzyme, making it a competitive biocatalyst and amenable to industrial applications. |
| Starting Page | 9363 |
| e-ISSN | 14220067 |
| DOI | 10.3390/ijms23169363 |
| Journal | International Journal of Molecular Sciences |
| Issue Number | 16 |
| Volume Number | 23 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-08-19 |
| Access Restriction | Open |
| Subject Keyword | International Journal of Molecular Sciences Agricultural Engineering Laccase Laccase Immobilization Corn Cob Lignocellulosic Biomass Dye Degradation |
| Content Type | Text |
| Resource Type | Article |
