NDLI: Formation of Lymphoma Hybrid Spheroids and Drug Testing in Real Time with the Use of Fluorescence Optical Tweezers

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Formation of Lymphoma Hybrid Spheroids and Drug Testing in Real Time with the Use of Fluorescence Optical Tweezers

Content Provider	MDPI
Author	Kamila, Duś-Szachniewicz Piotr, Ziółkowski Drobczyński, Sławomir Gdesz-Birula, Katarzyna Nowosielska, Emilia
Copyright Year	2022
Description	Interactions between stromal and lymphoma cells in the bone marrow are closely related to drug resistance and therapy failure. Physiologically relevant pre-clinical three-dimensional (3D) models recapitulating lymphoma microenvironmental complexity do not currently exist. In this study, we proposed a scheme for optically controlled hybrid lymphoma spheroid formation with the use of optical tweezers (OT). Following the preparation of stromal spheroids using agarose hydrogel, two aggressive non-Hodgkin lymphoma B-cell lines, Ri-1 (DLBCL) and Raji (Burkitt lymphoma), were used to conduct multi-cellular spheroid formation driven by in-house-developed fluorescence optical tweezers. Importantly, the newly formed hybrid spheroid preserved the 3D architecture for the next 24 h. Our model was successfully used for the evaluation of the influence of the anticancer agents doxorubicin (DOX), ibrutinib (IBR), and AMD3100 (plerixafor) on the adhesive properties of lymphoma cells. Importantly, our study revealed that a co-treatment of DOX and IBR with AMD3100 affects the adhesion of B-NHL lymphoma cells.
Starting Page	2113
e-ISSN	20734409
DOI	10.3390/cells11132113
Journal	Cells
Issue Number	13
Volume Number	11
Language	English
Publisher	MDPI
Publisher Date	2022-07-05
Access Restriction	Open
Subject Keyword	Cells 3d Lymphoma Model Optical Tweezers Single-cell Manipulations Cell Co-culture Cell Adhesion Lymphoma–stromal Cell Crosstalk Amd3100 (plerixafor) Doxorubicin (dox) Ibrutinib (ibr)
Content Type	Text
Resource Type	Article

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