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Evaluation of a Virus-like Nanoparticle Porcine Circovirus Type-2 (PCV2) Capsid Protein Fused with the Pig Immunoglobulin Fc Fragment as a Novel Vaccine Candidate against PCV2 in Mice
| Content Provider | MDPI |
|---|---|
| Author | Luo, Qingping Ahmed, Waqas Dai, Yichen Mohsin, Ali Hang, Haifeng Zhuang, Yingping Guo, Meijin |
| Copyright Year | 2021 |
| Description | Porcine circovirus Type 2 (PCV2) is a primary etiological pathogen of post-weaning multi-systemic wasting syndrome (PMWS). The capsid protein of PCV2 is the crucial immunogenic protein which can induce antibody generation and immune responses. However, there is still a lack of efficient PCV2 vaccines with high immunogenicity. In the current study, we developed a novel engineered PCV2 capsid (∆1-41aa)-pFc fusion protein (PCFP), which comprised a truncated capsid protein of PCV2 and a porcine IgG Fc fragment, fused to the capsid protein of PCV2 at the C-terminus. We found that this novel fusion protein could auto-assemble into virus-like nanoparticles with an estimated mean diameter of 22.6 nm, characterized by transmission electron microscopy. Immunization of BALB/c mice with this fusion protein significantly increased the production levels of anti-PCV2-capsid protein antibody in serum. Besides, the virus-like nanoparticles, PCFP was demonstrated to induce efficient cellular immune responses in mice, as evident by the high specific T cell reactivity to the PCFP fusion protein and the high production of the immune cytokines IFN-γ and IL-10 in an ex vivo re-stimulation system. Collectively, these findings demonstrate that the PCV2 truncated capsid subunit Fc-fusion protein can induce both cellular and humoral immune responses, and it displays great application potential. |
| Starting Page | 1128 |
| e-ISSN | 2076393X |
| DOI | 10.3390/vaccines9101128 |
| Journal | Vaccines |
| Issue Number | 10 |
| Volume Number | 9 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-10-03 |
| Access Restriction | Open |
| Subject Keyword | Vaccines Immunology Pcv2 Capsid (∆1-41aa)-pfc Fusion Protein Virus-like Nanoparticles |
| Content Type | Text |
| Resource Type | Article |
