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Silica Coating of Ferromagnetic Iron Oxide Magnetic Nanoparticles Significantly Enhances Their Hyperthermia Performances for Efficiently Inducing Cancer Cells Death In Vitro
| Content Provider | MDPI |
|---|---|
| Author | Cristian, Iacoviță Ionel, Fizeșan Nitica, Stefan Florea, Adrian Barbu-Tudoran, Lucian Dudric, Roxana Pop, Anca Vedeanu, Nicoleta Crisan, Ovidiu Tetean, Romulus Loghin, Felicia Lucaciu, Constantin Mihai |
| Copyright Year | 2021 |
| Description | Increasing the biocompatibility, cellular uptake, and magnetic heating performance of ferromagnetic iron-oxide magnetic nanoparticles (F-MNPs) is clearly required to efficiently induce apoptosis of cancer cells by magnetic hyperthermia (MH). Thus, F-MNPs were coated with silica layers of different thicknesses via a reverse microemulsion method, and their morphological, structural, and magnetic properties were evaluated by multiple techniques. The presence of a $SiO_{2}$ layer significantly increased the colloidal stability of F-MNPs, which also enhanced their heating performance in water with almost 1000 $W/g_{Fe}$ as compared to bare F-MNPs. The silica-coated F-MNPs exhibited biocompatibility of up to 250 $μg/cm^{2}$ as assessed by Alamar Blues and Neutral Red assays on two cancer cell lines and one normal cell line. The cancer cells were found to internalize a higher quantity of silica-coated F-MNPs, in large endosomes, dispersed in the cytoplasm or inside lysosomes, and hence were more sensitive to in vitro MH treatment compared to the normal ones. Cellular death of more than 50% of the malignant cells was reached starting at a dose of 31.25 $μg/cm^{2}$ and an amplitude of alternating magnetic field of 30 kA/m at 355 kHz. |
| Starting Page | 2026 |
| e-ISSN | 19994923 |
| DOI | 10.3390/pharmaceutics13122026 |
| Journal | Pharmaceutics |
| Issue Number | 12 |
| Volume Number | 13 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-11-27 |
| Access Restriction | Open |
| Subject Keyword | Pharmaceutics Oncology Iron Oxide Magnetic Nanoparticles Silica Coating Magnetic Hyperthermia Cancer Cells Alamar Blue Neutral Red A549 A35 |
| Content Type | Text |
| Resource Type | Article |
