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High-Throughput Continuous-Flow Separation in a Micro Free-Flow Electrophoresis Glass Chip Based on Laser Microfabrication
| Content Provider | MDPI |
|---|---|
| Author | Zhang, Aodong Xu, Jian Li, Xiaolong Lin, Zijie Song, Yunpeng Li, Xin Wang, Zhenhua Cheng, Ya |
| Copyright Year | 2022 |
| Description | Micro free-flow electrophoresis (μFFE) provides a rapid and straightforward route for the high-performance online separation and purification of targeted liquid samples in a mild manner. However, the facile fabrication of a μFFE device with high throughput and high stability remains a challenge due to the technical barriers of electrode integration and structural design for the removal of bubbles for conventional methods. To address this, the design and fabrication of a high-throughput μFFE chip are proposed using laser-assisted chemical etching of glass followed by electrode integration and subsequent low-temperature bonding. The careful design of the height ratio of the separation chamber and electrode channels combined with a high flow rate of buffer solution allows the efficient removal of electrolysis-generated bubbles along the deep electrode channels during continuous-flow separation. The introduction of microchannel arrays further enhances the stability of on-chip high-throughput separation. As a proof-of-concept, high-performance purification of fluorescein sodium solution with a separation purity of ~97.9% at a voltage of 250 V from the mixture sample solution of fluorescein sodium and rhodamine 6G solution is demonstrated. |
| Starting Page | 1124 |
| e-ISSN | 14248220 |
| DOI | 10.3390/s22031124 |
| Journal | Sensors |
| Issue Number | 3 |
| Volume Number | 22 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-02-01 |
| Access Restriction | Open |
| Subject Keyword | Sensors Analytical Chemistry Femtosecond Laser Microfabrication Glass Microfluidics Laser-assisted Chemical Etching Micro Free-flow Electrophoresis Continuous-flow Separation |
| Content Type | Text |
| Resource Type | Article |
