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Targeting the Mild-Hypoxia Driving Force for Metabolic and Muscle Transcriptional Reprogramming of Gilthead Sea Bream (Sparus aurata) Juveniles
| Content Provider | MDPI |
|---|---|
| Author | Fernando, Naya-Català Paula, Simó-Mirabet Jaume, Pérez-Sánchez Martos-Sitcha, Juan Heras, Verónica De Las Calduch-Giner, Josep |
| Copyright Year | 2021 |
| Description | On-growing juveniles of gilthead sea bream were acclimated for 45 days to mild-hypoxia (M-HYP, 40–60% O2 saturation), whereas normoxic fish (85–90% O2 saturation) constituted two different groups, depending on if they were fed to visual satiety (control fish) or pair-fed to M-HYP fish. Following the hypoxia conditioning period, all fish were maintained in normoxia and continued to be fed until visual satiation for 3 weeks. The time course of hypoxia-induced changes was assessed by changes in blood metabolic landmarks and muscle transcriptomics before and after exhaustive exercise in a swim tunnel respirometer. In M-HYP fish, our results highlighted a higher contribution of aerobic metabolism to whole energy supply, shifting towards a higher anaerobic fitness following normoxia restoration. Despite these changes in substrate preference, M-HYP fish shared a persistent improvement in swimming performance with a higher critical speed at exercise exhaustion. The machinery of muscle contraction and protein synthesis and breakdown was also largely altered by mild-hypoxia conditioning, contributing this metabolic re-adjustment to the positive regulation of locomotion and to the catch-up growth response during the normoxia recovery period. Altogether, these results reinforce the presence of large phenotypic plasticity in gilthead sea bream, and highlights mild-hypoxia as a promising prophylactic measure to prepare these fish for predictable stressful events. |
| Starting Page | 416 |
| e-ISSN | 20797737 |
| DOI | 10.3390/biology10050416 |
| Journal | Biology |
| Issue Number | 5 |
| Volume Number | 10 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-05-08 |
| Access Restriction | Open |
| Subject Keyword | Biology Hypoxia Hypo-metabolic State Growth Swimming Performance Metabolic Landmarks Muscle Transcriptome Glycolysis Lipid Metabolism Protein Turnover Gilthead Sea Bream |
| Content Type | Text |
| Resource Type | Article |
