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Raman Spectroscopy as Noninvasive Method of Diagnosis of Pediatric Onset Inflammatory Bowel Disease
| Content Provider | MDPI |
|---|---|
| Author | Acri, Giuseppe Venuti, Valentina Costa, Stefano Testagrossa, Barbara Pellegrino, Salvatore Crupi, Vincenza Majolino, Domenico |
| Copyright Year | 2020 |
| Description | We propose here a spectroscopic method to diagnose and differentiate inflammatory bowel diseases (IBD), such as ulcerative colitis (UC) and Crohn’s disease (CD) with pediatric onset, in a complete noninvasive way without performing any duodenal biopsy. In particular, the Raman technique was applied to proteic extract from fecal samples in order to achieve information about molecular vibrations that can potentially furnish spectral signatures of cellular modifications occurring as a consequence of specific pathologic conditions. The attention was focused on the investigation of the amide I region, quantitatively accounting the spectral changes in the secondary structures by applying deconvolution and curve-fitting. Inflammation is found to give rise to a significant increasing of the nonreducible (trivalent)/reducible (divalent) cross-linking ratio of the protein network. This parameter revealed an excellent marker in order to distinguish IBD subjects from non-IBD ones, and, among IBD patients, to differentiate between UC and CD. The proposed methodology was validated by statistical analysis using the receiver operating characteristic (ROC) curve. |
| Starting Page | 6974 |
| e-ISSN | 20763417 |
| DOI | 10.3390/app10196974 |
| Journal | Applied Sciences |
| Issue Number | 19 |
| Volume Number | 10 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2020-10-05 |
| Access Restriction | Open |
| Subject Keyword | Applied Sciences Analytical Chemistry Spectroscopy Inflammatory Bowel Disease Diagnosis Raman Spectroscopy Roc Curve |
| Content Type | Text |
| Resource Type | Article |
