NDLI: Comparison of the ColiPlate™ Kit with Two Common E. coli Enumeration Methods for Water

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Comparison of the ColiPlate™ Kit with Two Common E. coli Enumeration Methods for Water

Content Provider	MDPI
Author	Gibson, Cassi Maritim, Abraham Marion, Jason
Copyright Year	2021
Description	Quantitatively assessing fecal indicator bacteria in drinking water from limited resource settings (e.g., disasters, remote areas) can inform public health strategies for reducing waterborne illnesses. This study aimed to compare two common approaches for quantifying Escherichia coli (E. coli) density in natural water versus the ColiPlate™ kit approach. For comparing methods, 41 field samples from natural water sources in Kentucky (USA) were collected. E. coli densities were then determined by (1) membrane filtration in conjunction with modified membrane-thermotolerant E. coli (mTEC) agar, (2) Idexx $Quanti-Tray^{®}$ 2000 with the $Colilert^{®}$ substrate, and (3) the Bluewater Biosciences ColiPlate kit. Significant correlations were observed between E. coli density data for all three methods (p < 0.001). Paired t-test results showed no difference in E. coli densities determined by all the methods (p > 0.05). Upon assigning modified mTEC as the reference method for determining the World Health Organization-assigned “very high-risk” levels of fecal contamination (>100 E. coli CFU/100 mL), both ColiPlate and Colilert exhibited excellent discrimination for screening very high-risk levels according to the area under the receiver operating characteristic curve (~89%). These data suggest ColiPlate continues to be an effective monitoring tool for quantifying E. coli density and characterizing fecal contamination risks from water.
Starting Page	1804
e-ISSN	20734441
DOI	10.3390/w13131804
Journal	Water
Issue Number	13
Volume Number	13
Language	English
Publisher	MDPI
Publisher Date	2021-06-29
Access Restriction	Open
Subject Keyword	Water Water Science and Technology Fecal Indicator Bacteria Water Quality E. Coli Coliplate Colilert Environmental Microbiology
Content Type	Text
Resource Type	Article

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