NDLI: HepG2-NTCP Subclones Exhibiting High Susceptibility to Hepatitis B Virus Infection

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HepG2-NTCP Subclones Exhibiting High Susceptibility to Hepatitis B Virus Infection

Content Provider	MDPI
Author	Zahoor, Muhammad Atif Kuipery, Adrian Mosa, Alexander I. Gehring, Adam J. Feld, Jordan J.
Copyright Year	2022
Description	HepG2 cells reconstituted with Hepatitis B virus (HBV) entry receptor sodium taurocholate co-transporting polypeptide (NTCP) are widely used as a convenient in vitro cell culture infection model for HBV replication studies. As such, it is pertinent that HBV infectivity is maintained at steady-state levels for an accurate interpretation of in vitro data. However, variations in the HBV infection efficiency due to imbalanced NTCP expression levels in the HepG2 cell line may affect experimental results. In this study, we performed single cell-cloning of HepG2-NTCP-A3 parental cells via limiting dilution and obtained multiple subclones with increased permissiveness to HBV. Specifically, one subclone (HepG2-NTCP-A3/C2) yielded more than four-fold higher HBV infection compared to the HepG2-NTCP-A3 parental clone. In addition, though HBV infectivity was universally reduced in the absence of polyethylene glycol (PEG), subclone C2 maintained relatively greater permissiveness under PEG-free conditions, suggesting the functional heterogeneity within parental HepG2-NTCP-A3 may be exploitable in developing a PEG-free HBV infection model. The increased viral production correlated with increased intracellular viral antigen expression as evidenced through HBcAg immunofluorescence staining. Further, these subclones were found to express different levels of NTCP, albeit with no remarkable morphology or cell growth differences. In conclusion, we isolated the subclones of HepG2-NTCP-A3 which support efficient HBV production and thus provide an improved in vitro HBV infection model.
Starting Page	1800
e-ISSN	19994915
DOI	10.3390/v14081800
Journal	Viruses
Issue Number	8
Volume Number	14
Language	English
Publisher	MDPI
Publisher Date	2022-08-17
Access Restriction	Open
Subject Keyword	Viruses Virology Covalently Closed Circular Dna (cccdna) Hepatitis B Virus Hepg2-ntcp Cells Immunofluorescence Sodium Taurocholate Co-transporting Polypeptide (ntcp) Receptor Subcloning Limiting Dilution Myrcludex B
Content Type	Text
Resource Type	Article

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