NDLI: A Novel Fluorescent Aptasensor Based on Real-Time Fluorescence and Strand Displacement Amplification for the Detection of Ochratoxin A

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A Novel Fluorescent Aptasensor Based on Real-Time Fluorescence and Strand Displacement Amplification for the Detection of Ochratoxin A

Content Provider	MDPI
Author	Guo, Wei Yang, Haoyu Zhang, Yunzhe Wu, Hao Lu, Xin Tan, Jianxin Zhang, Wei
Copyright Year	2022
Description	It is urgently necessary to develop convenient, reliable, ultrasensitive and specific methods of ochratoxin A determination in food safety owing to its high toxicity. In the present study, an ultrasensitive and labeled-free fluorescent aptamer sensor combining real-time fluorescence with strand displacement amplification (SDA) was fabricated for the determination of OTA. In the presence of OTA, the OTA–aptamer combines with OTA, thus opening hairpins. Then, SDA primers specifically bind to the hairpin stem, which is used for subsequent amplification as a template. SDA amplification is initiated under the action of Bst DNA polymerase and nicking endonuclease. The amplified products (ssDNA) are dyed with SYBR Green II and detected with real-time fluorescence. The method has good linearity in the range of 0.01–50 ng $mL^{−1}$, with the lowest limit of detection of 0.01 ng $mL^{−1}$. Additionally, the fluorescent aptamer sensor shows outstanding specificity and reproducibility. Furthermore, the sensor shows excellent analytical performance in the artificial labeled detection of wheat and oat samples, with a recovery rate of 96.1~100%. The results suggest that the developed sensor has a promising potential application for the ultrasensitive detection of contaminants in food.
Starting Page	2443
e-ISSN	23048158
DOI	10.3390/foods11162443
Journal	Foods
Issue Number	16
Volume Number	11
Language	English
Publisher	MDPI
Publisher Date	2022-08-13
Access Restriction	Open
Subject Keyword	Foods Analytical Chemistry Ochratoxin A Strand Displacement Amplification (sda) Real-time Fluorescence Aptasensor
Content Type	Text
Resource Type	Article

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