NDLI: Evaluation of MDA-MB-468 Cell Culture Media Analysis in Predicting Triple-Negative Breast Cancer Patient Sera Metabolic Profiles

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Evaluation of MDA-MB-468 Cell Culture Media Analysis in Predicting Triple-Negative Breast Cancer Patient Sera Metabolic Profiles

Content Provider	MDPI
Author	Anna, Wróbel Marek, Bębenek Piotr, Młynarz Wojtowicz, Wojciech Pyziak, Karolina Tarkowski, Radosław Balcerzak, Alicja
Copyright Year	2020
Description	Triple-negative breast cancer (TNBC) is characterized by limited survival, poor prognosis, and high recurrence. Understanding the metabolic adaptations of TNBC could help reveal improved treatment regiments. Here we performed a comprehensive 1H NMR metabolic characterization of the MDA-MB-468 cell line, a commonly used model of TNBC, followed by an analysis of serum samples obtained from TNBC patients and healthy controls. MDA-MB-468 cells were cultured, and changes in the metabolic composition of the medium were monitored for 72 h. Based on time courses, metabolites were categorized as being consumed, being produced, or showing a mixed behavior. When comparing TNBC and control samples (HC), and by using multivariate and univariate analyses, we identified nine metabolites with differing profiles). The serum of TNBC patients was characterized by higher levels of glucose, glutamine, citrate, and acetoacetate and by lower levels of lactate, alanine, tyrosine, glutamate, and acetone. A comparative analysis between MDA-MB-468 cell culture media and TNBC patients’ serum identified a potential systemic response to the carcinogenesis-associated processes, highlighting that MDA-MB-468 cells footprint does not reflect metabolic changes observed in studied TNBC serum fingerprint.
Starting Page	173
e-ISSN	22181989
DOI	10.3390/metabo10050173
Journal	Metabolites
Issue Number	5
Volume Number	10
Language	English
Publisher	MDPI
Publisher Date	2020-04-27
Access Restriction	Open
Subject Keyword	Metabolites Biochemical Research Metabolomics Tnbc Mda-md-468 Triple-negative Breast Cancer 1h Nmr Spectroscopy
Content Type	Text
Resource Type	Article

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