NDLI: Sequential Hydrothermal HCl Pretreatment and Enzymatic Hydrolysis of Saccharina japonica Biomass

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Sequential Hydrothermal HCl Pretreatment and Enzymatic Hydrolysis of Saccharina japonica Biomass

Content Provider	MDPI
Author	Park, Eun-Young Park, Jung-Kyu
Copyright Year	2021
Description	This study investigated the production of fermentable sugars from carbohydrate-rich macroalgae Saccharina japonica using sequential hydrolysis (hydrothermal acid pretreatment and enzymatic hydrolysis) to determine the maximum reducing sugar yield (RS $y$ ). The sequential hydrolysis was predicted by three independent variables (temperature, time, and HCl concentration) using response surface methodology (RSM). Enzymatic hydrolysis (8.17% v/w $biomass$ $Celluclast^{®}$ 1.5 L, 26.4 h, 42.6 °C) was performed after hydrothermal acid pretreatment under predicted conditions (143.6 °C, 22 min, and 0.108 N HCl concentration). Using this experimental procedure, the yields of hydrothermal acid pretreatment, enzymatic hydrolysis, and sequential hydrolysis were 115.6 ± 0.4 mg/g, 117.7 ± 0.3 mg/g, and 183.5 ± 0.6 mg/g, respectively. Our results suggested that sequential hydrolysis of hydrothermal acid pretreatment and enzymatic hydrolysis was more efficient than their single treatment.
Starting Page	8053
e-ISSN	19961073
DOI	10.3390/en14238053
Journal	Energies
Issue Number	23
Volume Number	14
Language	English
Publisher	MDPI
Publisher Date	2021-12-01
Access Restriction	Open
Subject Keyword	Energies Paper and Wood Sequential Hydrolysis Hydrothermal Acid Pretreatment Enzymatic Hydrolysis Reducing Sugar Yield Biomass
Content Type	Text
Resource Type	Article

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