NDLI: Fluorescence Enhancement on Silver-Plated Plasma Micro-Nanostructured 3D Polymeric Microarray Substrates for Multiplex Mycotoxin Detection

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Fluorescence Enhancement on Silver-Plated Plasma Micro-Nanostructured 3D Polymeric Microarray Substrates for Multiplex Mycotoxin Detection

Content Provider	MDPI
Author	Koukouvinos, Georgios Karachaliou, Chrysoula-Evangelia Kanioura, Anastasia Tsougeni, Katerina Livaniou, Evangelia Kakabakos, Sotirios Petrou, Panagiota
Copyright Year	2021
Description	Oxygen plasma micro-nanostructured poly(methyl methacrylate) (PMMA) slides were modified through silver microparticle deposition to create microarray substrates that enhance the emitted fluorescence intensity. Silver deposition relied on a commercially available reagent and was completed in two 30-min incubation cycles of the substrate with the reagent. The fluorescence enhancement achieved using these substrates over flat PMMA slides was determined through the development of a microarray for the multiplexed detection of four mycotoxins, aflatoxin B1, ochratoxin A, fumonisin B1, and deoxynivalenol. It was shown that the implementation of silver-plated oxygen plasma micro-nanotextured PMMA substrates increased the signals obtained for aflatoxin B1 and ochratoxin A by approximately 2.8 times, 5.6 times for deoxynivalenol, and 16-times for fumonisin B1, compared to flat PMMA substrates. Most notably, this signal increase was not accompanied by a significant increase in the non-specific signal. In addition, the spot repeatability both across a single slide as well as between different slides was high, with coefficients of variation lower than 12%. The slides were also stable for at least three months, thus offering a microarray substrate with improved properties compared to standard glass slides, regarding both the absolute spot fluorescence intensity and between spots repeatability.
Starting Page	392
e-ISSN	22279717
DOI	10.3390/pr9020392
Journal	Processes
Issue Number	2
Volume Number	9
Language	English
Publisher	MDPI
Publisher Date	2021-02-21
Access Restriction	Open
Subject Keyword	Processes Analytical Chemistry Silver-enhanced Fluorescence Plasma Micro-nanostructured Substrates Microarrays Multiplexed Mycotoxin Detection
Content Type	Text
Resource Type	Article

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