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A Murine $CD8^{+}$ T Cell Epitope Identified in the Receptor-Binding Domain of the SARS-CoV-2 Spike Protein
| Content Provider | MDPI |
|---|---|
| Author | Yang, Jihyun Kim, Eunjin Lee, Jong-Soo Poo, Haryoung |
| Copyright Year | 2021 |
| Description | The ongoing COVID-19 pandemic caused by SARS-CoV-2 has posed a devastating threat worldwide. The receptor-binding domain (RBD) of the spike protein is one of the most important antigens for SARS-CoV-2 vaccines, while the analysis of CD8 cytotoxic T lymphocyte activity in preclinical studies using mouse models is critical for evaluating vaccine efficacy. Here, we immunized C57BL/6 wild-type mice and transgenic mice expressing human angiotensin-converting enzyme 2 (ACE2) with the SARS-CoV-2 RBD protein to evaluate the IFN-γ-producing T cells in the splenocytes of the immunized mice using an overlapping peptide pool by an enzyme-linked immunospot assay and flow cytometry. We identified SARS-CoV-2 $S_{395–404}$ as a major histocompatibility complex (MHC) class I-restricted epitope for the RBD-specific CD8 T cell responses in C57BL/6 mice. |
| Starting Page | 641 |
| e-ISSN | 2076393X |
| DOI | 10.3390/vaccines9060641 |
| Journal | Vaccines |
| Issue Number | 6 |
| Volume Number | 9 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-06-11 |
| Access Restriction | Open |
| Subject Keyword | Vaccines Immunology Sars-cov-2 Cell-mediated Immunity Cd8 Cytotoxic T Lymphocyte Epitope Vaccine |
| Content Type | Text |
| Resource Type | Article |
